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In contrast, there were almost no changes in the 1H-15N HSQC spectrum of SPOP MATH upon addition of Geminin SBC-alanine mutant peptide (Fig. 1i), demonstrating that the SBC motif 199VSSST203 is essential for SPOP-Geminin interaction. We also determined the X-ray crystal structure of SPOP...
Nuclear magnetic resonance (NMR) spectroscopy confirmed this binding motif and better defined the binding interface in SPOP. The Geminin WT SBC-containing peptide (195AEGTVSSSTDAKPCI209) and the SBC-alanine mutant counterpart (195AEGTVAAAADAKPCI209) were tested for interaction with the recombinant S...