FIP-fve為一個從針菇 (Flammulina velutipes) 純化出的免疫調節蛋白,被歸在真菌免疫調節蛋白 (FIPs),此的蛋白具有調控 IFN-γ 增加,減少過敏發炎,而對抗癌症功效的文獻較少.Rac1是一個小分子訊息傳遞 G 蛋白,與許多細胞的訊息調控,如細胞增生,細胞間的附著與細胞的移調控等.RACGAP1 (Rac GTP水解酶活化蛋白...
Characterization of an immunomodulatory Der p 2-FIP-fve fusion protein produced in transformed rice suspension cell culture Expression of FIP-fve gene in E.coli and preliminary study on its activity
FIP-fve為一種從金針菇 (Flammulina velutipes) 純化出來的免疫調節蛋白,具有刺激人類週邊血單核球細胞產生干擾素-γ的活性,經由蛋白質3D結構預測,發現FIP-fve的結構與澱粉酶之澱粉結合區的醣類結合模組相類似,這暗示著澱粉具有破壞FIP-fve刺激人類週邊血單核球細胞產生干擾素-γ的效果.本實驗藉由ELISA實驗觀察人類週...
FIP-fve is a member of the fungal immunoregulatory protein family. It was demonstrated to suppress systematic hypersensitive reaction and enhance cytokines IL-2 and IFN-gamma secretion by human peripheral blood mononuclear cells. We previously found the characteristic of FIP-fve induced lymphocytes act...
We found that FIP-gts exhibited better immunomodulatory activity than FIP-fve. Activities were both greatly reduced with duration of heating. For digestibility, FIP-fve was more resistant than FIP-gts to digestive enzymes in simulated gastric fluid and simulated intestinal fluid. IFN- production is...
aA new glycoprotein class was recently identified in Lingzhi and named fungal immunomodulatory proteins (FIPs). To date, four FIPs, termed LZ-8, FIP-fve, FIP-vvo, and FIP-gts, were purified from Ganoderma lucidum, Flammulina veltipes, Volvariella volvacea, and Ganodermn tsugae respectively. The...
郑中军目前担任青岛盛锦添防水工程有限公司法定代表人,同时担任青岛盛锦添防水工程有限公司执行董事兼总经理;二、郑中军投资情况:郑中军目前是青岛盛锦添防水工程有限公司直接控股股东,持股比例为100%;目前郑中军投资青岛盛锦添防水工程有限公司最终收益股份为100%;三、郑中军的商业合作伙伴:基于公开数据展示,郑中军与...
FIP-fve 為純化自金針菇之免疫調節蛋白,研究指出 FIP-fve 可刺激人類周邊血淋巴細胞增生及分泌 IFN-γ,並使免疫反應趨向於 TH1 反應;體內試驗亦發現,口服 FIP-fve 可降低小鼠食物過敏的症狀,延長罹癌小鼠之壽命及抑制腫瘤大小.生物可及性 (bioaccessibility) 是指活性成分自食物基質釋出後,可通過腸胃道的部分,...
Recombinant strain capable of expressing fungal immunomodulatory protein from Flammulina velutipes (FIP-fve), construction method, protein expression and purification method, and protein application一种能够表达金针菇免疫调节蛋白的重组菌株及其构建方法,蛋白表达纯化方法和蛋白应用,它涉及一种重组菌株的构建,表达...
(1%,V/V)为碳源进行诱导型表达4d达到最高,粗蛋白表达量为158.2 mg/L,在以葡萄糖(10%)和甘油(1%,V/V)为碳源进行组成型分别在表达第4天和第5天达到最高,粗蛋白分别为46.3 mg/L和29.5 mg/L.SDS-PAGE及Western blotting证明重组Fip-fve已正确表达,血细胞凝集活性检测初步证明重组Fip-fve具有良好生物...