Fibroinase, a cathepsin L-like cysteine protease, was previously identified in the silk gland of the silkworm, Bombyx mori. It shows high degradation activity during the pre-pupa period, when the silk gland undergoes apoptosis and remodeling. Here, we recombinantly expressed pro-fibroinase and ...
1. 1. High activity of fibroinase, an enzyme to hydrolyze the fibroin molecule, with complete hydrolysis of the heavy chain of fibroin within 1 hr in standard assay conditions, was demonstrated in extracted enzyme preparations from the silk gland in the fourth molt stage in the silkworm, ...
Compared with the prior art, the catalytic reinforcement method has the beneficial effects that (1) the appearances of silk fabric cultural objects are preserved by catalytic reinforcement of the fibroinase, and the strength and the compliance of the silk fabric are greatly improved; (2) the ...
1) Fibroinase 丝蛋白酶2) serine protease 丝氨酸蛋白酶 1. Cross-immunoreactivity between serine proteases from Anopheles dirus and Manduca; 大劣按蚊与烟草天蛾丝氨酸蛋白酶的交叉免疫活性 2. Cell microarray in detection of serine protease expression in Cryptococcus neoformans; 细胞芯片检测新生隐球菌...
cardiac scaffoldselectrospinningsilk‐fibroinSPIONMimicking the structure of extracellular matrix (ECM) of myocardium is necessary for fabrication of functional cardiac tissue. The superparamagnetic iron oxide nanoparticles (SPIONs, Fe3O4), as new generation of magnetic nanoparticles (NPs), are highly ...
1.1. The occurrence of an enzyme which hydrolyzes fibroin was demonstrated in degenerating silk gland in the pharate adult of the silkworm, Bombyx mori. The enzyme was named fibroinase.2.2. The assay system of fibroinase constructed in the present study consisted of tissue homogenate or its ...