1. For immunofluorescence, hanging culture membranes were fixed in 4% paraformaldehyde, permeabilized with 0.5% Triton X100, preabsorbed with 10% fetal calf serum, and incubated overnight at 4 °C with a combina
Serum biomarker panels for the diagnosis of gastric cancer. Wu, Zhang, Ma, Xu, Yang, Xu, Que, Chen, Xu, Cancer Med(2019 04) Adiposity Alters Genes Important in Inflammation and Cell Cycle Division in Human Cumulus Granulosa Cell. Merhi, Polotsky, Bradford, Buyuk, Chosich, Phang, Jindal...
Sf9 insect cells (VWR, EM71104-3) were maintained in ExCell 420 serum-free media (Sigma, 14420C). For baculovirus expression of HOOK3, pFastBac-M13-derived plasmids were transformed into DH10BacYFP competent cells and plated on LB agar supplemented with 30 μg ml−1 kanamycin (Sigma...
Sf9 insect cells (VWR, EM71104-3) were maintained in ExCell 420 serum-free media (Sigma, 14420C). For baculovirus expression of HOOK3, pFastBac-M13-derived plasmids were transformed into DH10BacYFP competent cells and plated on LB agar supplemented with 30 μg ml−1 kanamycin (Sigma...
SERUM GALECTIN-3 AND FIBROBLAST GROWTH FACTOR-23 LEVELS IN RELATION WITH TYPE 2 DIABETES AND CARDIOVASCULAR RISK Methods: A total of 35 T2D patients and 36 controls were consecutively enrolled. Serum galectin-3 and FGF-23 were determined by ELISA. The ASCVD risk ... A Kjisic,S Gluscevic,...
confirmed to be specific for FGF-13, as it was not observed when sections were stained with the same antibody in the presence of excess (approx. 10,000-fold molar concentration) epitope peptide Figure 3c, or when non-immune (normal) rabbit serum was used as the primary antibody Figure 3d...
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Serum HCG was measured by fluoro-immunometric assay. The data were analysed by paired two-tailed t-test, following logarithmic transformation. From days 1-5, there was an increase in serum B-LH (mean, 95% confidence intervals given in parentheses) from 8.3 (6.8, 10.2) IU/l to 11.7 (...
BCR was defined as serum prostate specific antigen level 0.2ng/mL or greater. Cox proportional hazards regression analyses of PSM lengths were conducted either as a continuous or categorical variable relative to location as a predictor of BCR. Results: Multivariate analyses demonstrated...
The mean serum thyroxine concentration was significantly decreased in the Tx (<1 渭g%) as compared to the C (14.2 渭g%). The combined heart and lung weights were significantly reduced in the Tx. Compared to the C lung, light and electron microscopic examination of the Tx lung revealed ...