FGFR3 Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请...
FGFR3 Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请...
Fig9: Flow cytometric analysis of A549 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary _x0018_?眝售后...
a, Immunoprecipitation using a pFGFR3 antibody. SUM185PE and MDA-MB-468 (negative control) cell lysates were subjected to immunoprecipitation with the pFGFR3 antibody and then Western blotted with the indicated antibodies. The arrow indicates the FGFR3-TACC3 fusion protein and the bracket ...
An irregular antibody screen on the patient yielded a positive result, and the presence of anti-Le antibodies was confirmed. Antibodies are found within the serum sample. A low titer anti-E antibody was detected via an enhanced test, following the transfusion reaction. The patient's red blood ...
a, Western blot analysis of phosphotyrosine immunoprecipitation of F3–T3;shTrp53 and HRAS(12V);shTrp53 mGSCs using the PIN4 antibody. F3–T3 and HRAS(12V) expression are shown. α-Tubulin is shown as a loading control. b, Immunofluorescence images using the phospho-PIN4(Y122)-specific an...
(mUC); however, over the last decade there have been significant advances in systemic therapy, which includes the FDA approval of the pan-FGFR inhibitor erdafitinib (approved for tumors with FGFR2/3 alterations) and the antibody drug conjugate (ADC) enfortumab vedotin (which targets the cell...
Sections were washed and incubated with secondary antibody Alexa Fluor 488 goat anti-rabbit (A11034, 1:1,000, Invitrogen), Alexa Fluor 488 donkey anti-goat (A11055, 1:1,000, Invitrogen), Alexa Fluor 546 goat anti-rabbit (A11056, 1:1,000, Invitrogen), Alexa Fluor 647 goat anti-rabbit...
Supernatant was harvested and an enzyme-linked immunosorbent assay (ELISA) performed using anti-human MIP-1α/CCL3 antibody (R&D Systems). Final MIP-1α concentration was corrected for cell viability at the end of the full culture period. The experiment was performed in triplicate. Gene-...
Anti-phosphotyrosine (PY99) antibody (Sanra Cruz, Dallas, TX, USA) (100 μL; 1:500, diluted in 5 mg/mL bovine serum albumin (BSA) T-PBS) was then added. After a 30-min incubation at 37 °C, the plate was washed three times, and 100 μL of horseradish peroxidase-conjugated goat...