Protein Construction: A DNA sequence encoding the amino acids (Gln38-Ser208) of human FGF10 (Accession Number: O15520) was expressed. Synonyms: Fibroblast growth factor 10; Keratinocyte growth factor 2; KGF2; KGF-2. Purity: ≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Co...
纯化方法:affinity purified by Protein A交叉反应:hu, mo, rat, Rb, hrs, cow, shp, dog, chk, Gpig产品介绍:FGF10/FGF-10 (fibroblast growth factor 10) is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF10 binds the KGF...
Effect of FGF10 on adipogenesis was investigated by gaining and losing function of FGF10 in vitro. And then, we examined several lipid metabolism-related genes, including peroxisome proliferator activated receptor γ (PPARγ), sterol regulatory element binding protein 1 (SREBP1), preadipocyte factor...
How thermodynamic stability contributes to the function of FGFs during development remains unknown. Here we show that FGF10, an important limb and lung morphogen, exists as an intrinsically unstable protein that is prone to unfolding and is rapidly inactivated at 37掳C. Using rationally driven ...
However it is important to remember this analysis does not rule out a function for the other Gata3 binding sites in Fgf10 regulation, merely that they are not necessary for reporter activity. Nevertheless the relatively poor affinity for binding the GATA3 protein does suggest they are probably ...
Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with... During the initial stages of metanephric development, low levels of SHH, Fgf8, and Fgf10 expression were observed, which were found to increase...
We found that Merlin protein levels as well as Mst1/2 phosphorylation are decreased (Figures 2H and 2I), while nuclear Yap and Wnt7b are increased in secretory and ciliated daughter cells of Scgb1a1-Ilkf/f mice (Figures 2D–2G). Importantly, we found a significant increase in Fgf10 ...
Our results showed that none of these variants affected the intracellular localization, and both the wild-type and mutated proteins were expressed in the cytoplasm (Figs. 3e, 4e). Thus, we hypothesized that the mutations might change the protein function through other mechanisms....
(1). Mature mouse FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). C-terminal to this region (aa 62‑209), it shares 94% and 100% amino acid sequence identity with human and rat FGF-10, respectively. FGF-10 is secreted ...
development.Fgf7may have some roles in the invagination of the epithelium, whileFgf10may support the proliferation of the nascent superficial gland cells and the direct elongation and proliferation of the invaginated epithelia, and the HG primordia makes the FGF10 protein gradient in the periocular...