qRT–PCR analysis Fresh-frozen tissue cDNA prepared from RNA extracted from fresh-frozen tumours was analysed on Format 32 384-well Taqman Low Density Array (TLDA) cards (Applied Biosystems, Warrington, UK), designed such that the expressions of 32 genes (31 target genes and 18S ribosomal RNA...
2.1. Preparation and fabrication of the PCL/nHA/collagen scaffold with dual growth factors (FGF-2 and BMP-2) Commercial PCL (Mw= 43,000–50,000 Da, Polysciences, Warrington, PA, USA) and nHA (particle size: < 200 nm, Sigma-Aldrich, St. Louis, MO, USA) were purchased to prepare the...