Assessing the amount of FFPE tissue needed for adequate RNA extraction While FFPE tissues are more abundant than fresh tumor tissue, these archival tissues still represent a limited resource that require judicious utilization. Thus, we initially set out to determine the minimum amount of FFPE breast...
尽管FFPE保存样本对生命科学研究来说是革命性的,但这种处理方式会破坏DNA/ RNA分子,基本上会出现严重的降解情况。甲醛固定和组织包埋实验会降解FFPE组织中的RNA(Evers DL, et al. 2011),形成高度碎片化的低完整性的RNA分子,通常RNA完整性指数(RIN)<2.5(Webster AF, et al. 2015)。这让转录组层面的依赖于逆转录...
RNA deparaffinisation, extraction and purification were performed according to the High Pure FFPE RNA Micro Kit (Roche, Mannheim, Germany) protocol. RNA concentration and Å260/Å280 ratio were determined using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA...
Moreover, global gene expression analysis of RNA and whole-genome copy number analysis of DNA can be successfully applied to FFPE tissues processed with the proper procedures (Gunn et al., 2018; Wimmer et al., 2018). Show abstract OpenMS – A platform for reproducible analysis of mass ...
Multiplex tissue proteomic assays using FFPE IHC analysis Fast Detection and Differentiation of KPC and MBL Carbapenemases among Enterobacterales Isolates by a Modified Combined-Disk Test This review was directed to foster a modest, fast, and precise altered consolidated plate test (mCDT) way to deal...
Our results suggest a minimum concentration of 25 ng/ul FFPE-extracted RNA for library preparation and 1.7 ng/ul pre-capture library output to achieve adequate RNA-seq data for downstream bioinformatics analysis. Peer Review reports Background For decades, clinical biospecimens have been typically ...
Renal amyloidosis typically manifests albuminuria, nephrotic-range proteinuria, and ultimately progresses to end-stage renal failure if diagnosed late. Different types of renal amyloidosis have completely different treatments and outcomes. Therefore, amy