Beijing, China). Equal amounts of RNA were reverse transcribed using HiFiScript cDNA synthesis kit according to the manufacturer’s instructions (CWBIO, Beijing, China) in 8-strip tubes (#404001, Nest, China). The real-time polymerase chain reaction was carried out using SYBR Green PCR Matser...
One microgram of RNA was reverse transcribed into cDNA using a first strand cDNA synthesis kit (Toyobo, Osaka, Japan) according to the manufacturer’s instructions. RNA extraction and reverse-transcription were performed as described previously [30]. qRT-PCR was performed on an ABI StepOne Plus ...
Reverse Transcription-Quantitative PCR RNA was extracted using the Qiashredder and QIAGEN RNeasy Mini kits (QIAGEN) according to the manufacturer's protocol. 2 μg total RNA for each sample was used as input for each reverse transcription reaction, performed using the TaqMan RT kit (Applied Biosys...
During infection the host relies on pattern-recognition receptors to sense invading fungal pathogens to launch immune defense mechanisms. While fungal recognition and immune effector responses are organ and cell type specific, during disseminated candidi
Linear trajectory interference analysis was performed using the SCORPIUS algorithm and locally weighted smoothing regression was performed for the visualization of gene or KEGG pathway expression along the established pseudotime trajectory. These single-cell data have been deposited in the Array Express ...
2.17. Quantitative PCR Total RNA in cells or tissues was extracted using RNA-Quick Purification Kit (YiShan Biotechnology, Shanghai, China). Then cDNA was transcribed from RNA with PrimeScript RT Master Mix (Takara, Japan). Quantitative PCR was performed using SYBR Green Mix (CwBio). The primer...
RNA (1 μg) was transcribed to cDNA with a Reverse Transcriptase transcription kit (Transgene). Quanti- tative real-time PCR was performed with a qPCR kit for SYBR Green I (Transgene) in a LightCycler 96 system (Roche). β-actin was used as the housekeeping gene. The relative gene ...
Overall RNA from the whole frozen cardiac tissues and iced cell were extracted by Trizol (Invitrogen), and reversely transcribed into cDNA by a cDNA synthesis kit (Takara Clontech, Dalian, Japan). As quantitative PCR was performed by a Step one plus Real-Time PCR system (Applied Biosystems, ...
(Keap-1) into the proteasome degradation pathway. Under stressful conditions, Nrf2 translocates to the nucleus to instigate the transcription of antioxidant response element-containing genes. Moreover, stimulation of Nrf2 can negatively modulate ferroptosis through iron, glutathione, lipid metabolism as ...
Within this array of cytokines, IFN-γ has been empirically shown to instigate ferroptosis in salivary gland epithelial cells (SGECs) in SS, consequently compromising salivary gland function via activation of the JAK/STAT1 pathway [10]. IL-7 activates IFN-γ and promotes the interaction between ...