Size distribution and surface charge of the prepared protein nanoparticles were measured in triplicate using a Zetasizer Nano ZS (Malvern Instruments,Ltd., Worcestershire, UK) equipped with a 633 nm wavelength of laser. Each sample was dissolved in distilled water at concentration of 1.0 mg/m...
aeruginosa PAO1 is performed by a heterooligomeric bacterioferritin, where the relative content of FtnA and BfrB subunits is strongly affected by the environmental O2 levels. The heterooligomeric Bfr in P. aeruginosa is similar to the heterooligomeric mammalian Ftn assembled from H and L subunits...
0.016) compared with those with a lower average in a univariate analysis of a UK population with β-thalassemia major [17]. The multivariate analysis in the same study controlled for age, sex, and worst liver T2* and showed that average 10-year SF levels >1250 μg/L were significantly as...
The four HFn-based proteins were first labelled by Sulfo-cy5 NHS ester (Lumiprobe, Hunt Valley, MD, USA) with a molar ratio of 1:30 (Protein to Cy5) and the uncoupled Cy5 was removed by Hitrap G25 desalting chromatography. As 4T1 is a BALB/c breast tumor cell line, female BALB/c ...