1. A human stem cell feeder layer cell line comprising an expression vector encoding senescence-triggering factors. 2. The cell line of claim 1, wherein the senescence triggering factors are Cip/Kip cyclin-dependent kinase inhibitor family members p21, p27, or p57 3. The cell line of claim...
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Using our defined extracellular matrix and low oxygen culturing conditions for the differentiation and maturation of human cortical neurons, we further modified neuronal progenitor cell seeding densities and feeder layer-free culturing conditions in miniaturized formats (i.e., 96 well) to decrease ...
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Finally, I describe our use of a bioreactor to scale up the production of the stem cells in order to obtain enough hepatocytes which can be used for liver cell transplantation and bio-artificial liver support devices, as well as for toxicology and pharmacology studies.Hasan, Ahmad S...
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