Fed-batch fermentation (FBF) is combination of batch and continuous mode where substrate is added into the reactor without removing the medium to avoid the problem of substrate inhibition (Azhar et al., 2017). From: Bioresource Technology, 2022 ...
2007. "Biosorption of Copper(II) Ions onto Powdered Waste Sludge in a Completely Mixed Fed-Batch Reactor: Estimation of Design Parameters". Bioresource Technology. 98: 1155-1162.Y. Pamukoglu, F. Kargi, Biosorption of copper(II) ions onto powdered waste sludge in a completely mixed fed-batch...
batch culture were subjected to RNASeq. To understand the contribution of mRNA expression levels towards the observed fed-batch titer and productivity, we calculated the FPKM of each of the product mRNAs relative to the housekeeping gene β-actin (Fig.1f). The IgG1producer exhibited higher expres...
Large-scale manufacturing was completed using a 50-L Marubishi fermentor; relevant reactor geometric data are shown in Table I. The height of the liquid was calculated ifor the Marubishi 5-L fermenter assuming a flat bottom using equation (1) and for a 50-L fermenter with an ell...
Equation (1) assumes a constant biomass yield on the growth limiting substrate and a constant value of the maintenance coefficient. During growth at a constant specific growth rate μ, the total amount of biomass in the fermenter, i.e., X t V t , increases with time as follows: $$X_{...
1.21.3.1Feed Medium Design Medium development for a fed-batch process typically includes three aspects, the formulations of basal medium and feed medium, and the optimization of feeding strategy. For the detailed selection of an appropriate basal medium for a specific cell line, the reader is refe...
Depending on this equation, to produce 1 mol of biomass, 0.94 moles of oxygen need to be utilized. 3.4. Batch operation 3.4.1. Mixing To achieve uniform mixing in the laboratory-built membrane bioreactor, a shaker and a pump were compared. Their effectiveness was tested indirectly by ...
BES performance can be affected by several biological, operational and design parameters [15]. Types of microorganisms (electroactive or not) and substrate, mechanisms of extracellular electron transfer, electrodes characteristics, reactor geometry and flow rates, external resistances and other variables mus...
The fed-batch process is formulated as a nonlinear switched time-delay system. In general, the time-delay in the fed-batch process cannot be exactly estimated. Our goal is to design an optimal switching control scheme to simultaneously maximize 1,3-PD productivity and 1,3-PD yield under ...
Optimisation of the GFB phase of fed-batch culture to improve volumetric FFase activity by the GAP strain was therefore worth investigating, to assess the extent by which enzyme production could be enhanced. The results from the central composite design (CCD) where volumetric FFase activity (Uf)...