Eleven of fifteen contained lymphocytes, both B cells (CD20 positive) and T cells (CD3 positive). In 20% of the samples there was evidence of ectopic lymphoid like structures with focal aggregations of B cells and T cells, segregated into anatomically different adjacent zones. To investigate ...
The human T-cell lymphotropic virus type I (HTLV-I) is a retrovirus that was first isolated in 1981 from T-cells of a patient with cutaneous lymphoma, and was soon after linked to a type of leukemia/lymphoma that had been previously described in Japan, adult T-cell leukemia/lymphoma (ATL...
1f). While different tumor subtypes (SCLC-A, -N, -P) displayed close interactions among themselves, they exhibited varying levels of epithelium–immune crosstalk with B cells, T cells, neutrophils, DCs, and plasma cells (Fig. 1f). Particularly noteworthy was the strong association of all ...
Transplantation of human cells Frozen human PBMCs were purchased from STEMCELL Technologies (Cancouver, BC, Canada). CD8+ T cell-depleted hPBMCs were obtained using magnetic beads coated with anti-human CD8 antibody with a MACS column (Miltenyi Biotec Japan, Tokyo, Japan), according to the man...
For double stainings of T cells and LP cells either CD20 and CD3 were applied as double staining or PD1 and CD79a or CD8 and CD79a. As secondary antibodies, the VectaFluor Duet Immunofluorescence Double Labeling Kit DyLight 488/594 (Vector Laboratories, Burlingame, CA, USA, Cat. No. DK-...
To enable high-parameter phenotyping of antigen-specific cells, we modified a recently developed human T cell CyTOF panel21 so it would detect cells producing the cytokines IFNγ, IL-4, or IL-17 (Table S2). CD4+ and CD8+ T cells were identified by sequential gating on live, singlet CD3...
Immunohistochemical staining of CD4, CD8, CD163, IL-6, and MMP-11 was conducted using the following primary antibodies: monoclonal rabbit anti-human CD4 (clone SP35, catalog number: 7,904,423; Ventana Medical Systems, Tucson, AZ, USA), monoclonal mouse anti-human CD8 (clone C8/144B, ...
cellspartially expressedT—cell differentiatedantigens(CD3e,CD4,CD8)andNKcellassociatedantigen(CD56).rI1}1epositivefrequencyof CD3￡,CIM,CD8andCD56was28/42(66.7%),7/42(16.7%),10/42(23.8%)and12/42(28.6%) respectively.Thirty—ninecells(92.9%)expressedTIA.1,butnoneexpressedCD20andCD68.Morethanha...
cells, a subset of exhausted CD8+T cells, are revealed to highly infiltrate in pre-treatment tumors and show prominent progenitor exhaustion phenotype in post-treatment samples from responders. We validate CXCL13+CD8+Tex cells as a predictor of improved response to nICT and reveal CXCL13 to ...
The functions of the tumor microenvironment (TME) are orchestrated by precise spatial organization of specialized cells, yet little is known about the multicellular structures that form within the TME. Here we systematically mapped TME structures in situ