After dual PBS washes, 2 × 105 cells were incubated with 1% bovine serum albumin buffer (with Fc blocker, BioLegend, USA) for 0.5 h. Fluorochrome-labeled antibodies against an array of markers (CD45, CD38, CD138, and CD69) were obtained from BioLegend. Annexin V/FITC and 7AAD st...
2). However, this staining was blocked in the presence of FcR-bindable Ab (4G2) as well as FcR-blocker, demonstrating that staining with the irrelevant anti-TCR Ab is FcR-dependent and nonspecific. In staining with the irrelevant anti-TCR Ab, we pre-added Cas or Imd Ab to the cells....