Based on our earlier work, we devised an affinity tag by addition of two cysteine residues onto the N- and C-termini of the Fc-III peptide and designated as the Fc-III-4C tag, in which four cysteine residues form two disulfide linkages. The binding affinity of Fc-III-4C tag to human...
Here by analyzing the expression of different FcRs in intestinal ILCs, we find that ILC3s highly express Fcer1g and Fcgr3, the latter encoding CD16 (also known as FcγRIII). FcεR1γ is dispensable for ILC3 development and their transcriptional states. However, it maintains the protein ...
Co-crystals of SEFL2.2 Fc were generated in complex with a minimized Z-domain (Mini-Z) peptide. This engineered two-helix bundle of 34 amino acids binds tightly to the CH2-CH3 hinge region in the Fc and serves to optimize Fc intramolecular contacts, improving the conditions for crystal ...
In contrast, binding of IgG-Fc to its homologous low-affinity receptor, FcγRIII, exhibits a smaller change in heat capacity [Math Processing Error]. Fluorescence energy transfer experiments have shown that the average distance between the N and C termini of the IgE is only ∼70 Å, a ...
FcB1-schizont-ESTs matching the mitochondrial genome [UniProt:AJ276844] were mapped at the level of the three described genes: putative cytochrome oxidase III [UniProt:Q9MDY3], putative cytochrome oxidase I [UniProt:Q02766] and putative cytochrome b [UniProt:Q02768]. The single EST matching ...
As expected, no Fc peptide glycans could be detected for the FcG− B-cell lines (Fig. 2a, c). The glycans expressed in the non-mutated ɣHC of the IgG-BCRs were mainly di-antennary (A2) complex-type glycans containing core fucosylation, 77% (2G9 IgG-BCR), 88% (3F3 IgG-BCR...
图4c显示了当在吞噬作用测定之前将mc38癌细胞与2.5f-fc和抗cd47抗体一起预孵育时吞噬癌细胞的巨噬细胞的百分比的增加(28.4%)。[0325]测试了抗cd47抗体和2.5-fc对其他癌细胞(b16f10、e0771、4t1和u87mg(其为人恶性胶质瘤细胞系))和非癌性细胞293t的组合效应。如图5a和图5b所示,与单独的抗cd47抗体或2.5-fc...
Finally, to further assess immunogenicity, NetMHC4.1 was used to predict T-cell epitopes and IEDB analysis was used to predict antibody epitopes, in which the REW amino acid substitutions did not significantly increase the number of potentially immunogenic peptides or epitopes compared with WT IgG1...
Strong evidence supports that B cells play a vital role in RA, as large amounts of autoantibodies are produced in RA patients, including rheumatoid factors (RF), anti-cyclic citrullinated peptide antibodies (ACPA) and type II collagen antibodies, and these antibodies are closely related to RA [...
The relative binding activity to FcγRIIB versus another Fc receptor (e.g., FcγRIII) of such an Fc variant is higher than the relative binding activity to FcγRIIB versus the other Fc receptor (e.g., FcγRIII) of the wild-type counterpart. The Fc variant may have enhanced binding...