pFUSE-hIgG1e1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain mutated T250Q/M428L and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion prote...
fusion::vector<int,std::string,float> seq{1,"abc",3.4f}; fusion::vector<std::string,std::string,std::string> strings=fusion::transform(seq,to_string);static_assert(strings==funsion::make_vector("1"s,"abc"s,"3.4"s)); AI代码助手复制代码 发生在第四象限上的计算,是类型计算,使用类型容...
auto to_string=[](auto t){ std::stringstream ss; ss<<t;returnss.str();};fusion::vector<int,std::string,float>seq{1,"abc",3.4f}; fusion::vector<std::string,std::string,std::string>strings=fusion::transform(seq,to_string); static_assert(strings==funsion::make_vector("1"s,"abc"...
The resultant gene was inserted into a pCAG-Hyg human IgG-Fc fusion vector (Fujifilm Wako) to generate the scR2agoTNF-Fc expression vector. Expression and purification of TNF‑α mutein. scR2agoTNF-Fc protein was expressed using the Expi293 Expression System (Thermo Fisher Scientific, ...
activity and an antibody biological effect function are combined together; and the Fc fusion protein-containing yeast expression vector is capable of directly constructing a protein gene with biological activity into the Fc end-containing fusion protein efficiently expressed in the expression vector.万晓春...
fc fusion protein ‑ dependent cellular cytotoxicity,pdcc)在肿瘤治疗中的应用。 11.为达到此发明目的,本发明采用以下技术方案: 12.第一方面,本发明提供了一种多肽 ‑ fc融合蛋白,所述多肽 ‑ fc融合蛋白包括:多肽和fc片段。 13.其中,所述多肽为特异性靶向肿瘤细胞的多肽,且所述多肽含有5 ...
We constructed the eukaryotic expression vector of human IL-35-IgG4 (Fc)-pOptiVEC?-TOPO? by gene recombination technique and expressed the fusion protein human IL-35-IgG4 (Fc) in CHO/DG44 cells. The two components of the newly discovered cytokine human IL-35, EBI3 and IL-12p35, were ...
本 发明还提供了一种多肽‑Fc融合蛋白依赖的NK细胞介导的细胞毒性作用(Peptide ‑Fc fusion protein‑Dependent Cellular Cytotoxicity,PDCC)在肿瘤治疗中的应用。 [0011] 为达到此发明目的,本发明采用以下技术方案: [0012] 第一方面,本发明提供了一种多肽‑Fc融合蛋白,所述多肽‑Fc融合蛋白包括:多肽 和Fc...
The DNA pools obtained from the elution after nine and after ten rounds were cloned into the TOPO™ TA®, pCR2.1 cloning vector (Invitrogen, Carlsbad, Calif.) and transformed intoE. coli. Between 30 and 100 clones were picked and grown into plasmid minipreps (Qiagen). Thirty-eight clone...
Disclosed are nucleotide sequences, for example, DNA or RNA sequences, which encode an immunoglobulin Fc-Leptin fusion protein. The nucleotide sequences can be inserted i