FBXW7 Rabbit Monoclonal Antibody -20ºC保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验...
FBXW7 Antibody (MA5-26562) in WB Western blot analysis of FBXW7 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with FBXW7 (Product # MA5-26562) monoclonal antibody. View...
FBXW7 primary antibody included. 推荐稀释比 应用推荐稀释比 Western Blot (WB) WB : 1:1000-1:4000 Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC) IHC : 1:50-1:500 Immunofluorescence (IF)/ICC IF/ICC : 1:50-1:500 It is ...
Invitrogen Anti-FBXW7 Polyclonal, Catalog # PA1-4253. Tested in Western Blot (WB) applications. This antibody reacts with Human samples. Supplied as 100 µg purified antibody (1 mg/mL).
FBXW7 Polyclonal Antibody查看其他16个FBXW7抗体 说明书 实验方案 技术支持 货号PA5-29193 此产品已停产 100 µL 抗体检测数据 (1) Application 图: 1 / 1 FBXW7 Antibody (PA5-29193) in WB Western blot analysis of Cdc4 using 30 µg of Raji lysate. Samples were loaded onto a 7.5...
Western blot analysis was performed to assess protein levels. Cell proliferation assays and FACS were performed determine cell proliferation and viability. A tissue microarray was used for immunohistochemical staining using FBxW7 antibody. Tissue stains were scored manually. Staining intensities were ...
While the intrapulmonary airway epithelium is normal in morphology in the mutant, we also assayed for cell type composition. TheFbxw7mutant lung showed increased club cells and decreased ciliated cells, as indicated by SCGB1A1 and FOXJ1 antibody staining (SupFig. 3A and B). There is no chang...
Finally, the supernatant was collected by centrifugation for western blot analysis. Ubiquitin pull-down NCI-H1299 cells were transfected with oe-NC or oe-FBXW7, and total cell lysates were collected by the above method. An equal amount of SOX9 antibody (ab185230, 1:60, Abcam) was added ...
C RT-PCR analysis and western blot analysis were used to detect the mRNA and protein expression level of RANBP10 in RANBP10-knockdown and control cells. D Cell proliferation of RANBP10-knockdown and control cells were examined by MTT assay. E BrdU staining assays were performed to detect ...
Cell lysates were immunoprecipitated with anti-Flag M2 agarose beads and immunoblotted with anti-phosphorylated-threonine (p-Thr) antibody. e HEK293T cells were co-transfected with Flag-FASN and HA-FBXW7β. Where indicated, CHIR (4 μM) was added for 12 h or 24 h before harvesting...