Compared to phosphate buffer saline (PBS) and normalized to RPMI 1640 medium, significant cytotoxicity was observed by 24 h in medium containing 50% AU and by 7 days in medium containing 30% AU. Live/Dead staining confirmed proliferation results and no pH-changes could be observed. Here we ...
主要成分为磷酸盐缓冲液(PBS),叠 氮钠(NaN3)和胎牛血清(FBS),FBS作为一种蛋白存在于缓冲液中可以减少抗体Chemicalbook的非特异性结合,叠氮钠可以维持细胞表面抗原的稳定性。配制溶液均经过无菌过滤处理并经过严格的质量控制。应用可用作荧光染料的稀释液以及流式分析用细胞的重悬、洗涤以及储存。主要应用于流式、直接...
Compared to phosphate buffer saline (PBS) and normalized to RPMI 1640 medium, significant cytotoxicity was observed by 24 h in medium containing 50% AU and by 7 days in medium containing 30% AU. Live/Dead staining confirmed proliferation results and no pH‐changes could be observed. Here we ...
91000 SMM0207 www.vishay.com Vishay Draloric TEST PROCEDURES AND REQUIREMENTS IEC EN 60068-2 (1) 60115-1 TEST CLAUSE METHOD TEST Stability for product types: SMM0207 4.22 4.40 4.17 4.18 REQUIREMENTS PERMISSIBLE CHANGE (ΔR) PROCEDURE STABILITY CLASS 0.25 OR BETTER STABILITY CLASS 0.5 OR BETTER ...
91000 SMM0207 www.vishay.com Vishay Draloric TEST PROCEDURES AND REQUIREMENTS IEC EN 60068-2 (1) 60115-1 TEST CLAUSE METHOD TEST REQUIREMENTS PERMISSIBLE CHANGE (ΔR) PROCEDURE Stability for product types: SMM0207 4.37 67 (Cy) Damp heat, steady state, accelerated (85 ± 2) °C (85 ± 5...
The human umbilical cord tissues were processed within 12 h of sample collection by the two most commonly used approaches as schematically outlined in Fig.2a. Cords were washed in 1X PBS containing antibiotics thrice to remove the blood clots. For the explant method, cord tissue was minced ...
PBS 1.20±0.17 0.0 aP<0.05, vs LacZ, PBS groups; cP<0.05, vs Bax group. Table 2 Cell growth inhibition of recombinant adenovirus on HT29 cell when combined with Ad/PGK-GV16 Group OD (x±s) Cell growth inhibition rates (%) Ad/GT-TRAIL+Ad/PGK-GV16 0.18±0.03ac 85.2 Ad/GT-Bax +...
The sections were then incubated in the blocking solution (PBS containing 10% goat serum, 5% milk, and 4% bovine serum albumin (BSA)) for 1 h at room temperature and treated with the primary antibody solution (PBS containing 0.1% Tween-20 and 2% BSA) for overnight at 4?C in ...
-labeled probes and biotin-labeled probes were diluted 200 times with 1% blocking regent in TNB), which was directly applied to the nuclei. After washing with wash buffer (0.05% Tween 20 in TNB) containing DAPI (0.1 µg/mL) for 10 min, the nuclei were washed once with PBS for...
91000 SMM0207 www.vishay.com Vishay Draloric TEST PROCEDURES AND REQUIREMENTS IEC EN 60068-2 (1) 60115-1 TEST CLAUSE METHOD TEST REQUIREMENTS PERMISSIBLE CHANGE (ΔR) PROCEDURE Stability for product types: SMM0207 4.37 67 (Cy) Damp heat, steady state, accelerated (85 ± 2) °C (85 ± 5...