Analysis of fatty acid methyl esters with high accuracy and reliability. II. Methylation of fats and oils with boron triflouride-methanol. I Chromatogr 1982; 247: 63-69.Bannon, C.D., Craske, J.D., and Hilliker, A.E. (1985) Analysis of Fatty Acid Methyl Esters with High Accuracy and...
Enrichment in peri-LD cages is an evolutionarily conserved property of seipin.aThe SEIP-1::Venus protein was stably expressed in COS7 cells. The cells were incubated with oleic acid, fixed and immunostained for endogenous PDI (the ER) and TOM20 (mitochondria). Alexa Fluor (AF)-405 and 594...
Fatty acid desaturase (FADS2) encoding Δ6 desaturase is a rate limiting enzyme for polyunsaturated fatty acid (PUFA) synthesis in liver. Adult offspring of mothers fed with fish oil or butter during pregnancy to weaning showed up-degrees of promoter's methylation and down-expression of FADS2,...
(FAMEs), samples were prepared as previously detailed24. In short, the chloroform phase was evaporated to dryness under nitrogen at 37 °C. Then samples were treated for 2 h at 100 °C by acidic methylation (1 mL methanol and 50 μL sulfuric acid). FAMEs were isolated through...
Tree peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of α-linolenic acid (ALA) for seed oil production and development in China. However,
没有方向什么我做? [translate] akeep current 保留潮流 [translate] aLipids were assayed gravimetrically, by analysis of fatty acid methyl esters released by transmethylation, or by ESI-MS 油脂检验测定重量,由对transmethylation发布的脂肪酸甲基酯的分析,或者被ESI-MS [translate] ...
soybean oil has been commonly used as part of the feed composition for growing-finishing pigs because it results in improved growth performance and beneficial effects to consumers [12]. In addition, soybean oil is high in polyunsaturated fatty acids (PUFA), being rich in linolenic acid (LA, C1...
(storage organs). Important regulators, functional categories, and differentially expressed genes involved in avocado fruit ripening were identified. Additionally, to demonstrate the utility of the avocado gene expression atlas, we investigated the expression patterns of genes implicated in fatty acid ...
Fatty acid profiles of mouse diets, tail and intestinal tissues were analyzed by gas chromatography as described previously60. Briefly, tissue or food samples were grounded to powder under liquid nitrogen and subjected to total lipid extraction and fatty acid methylation by 14% boron trifluoride (BF3...
Fatty acid extraction, methylation and analysis on gas chromatograph (GC) was performed as described in Steinrücken et al. [14]. The concentration of internal standard (23:0 FAME dissolved in isooctane) was adjusted for the respective DW of the samples, with 100, 240, 380, and 480 μg ...