Each fatty acid methyl ester was detected in the selected-ion monitoring mode. All results were normalized to the peak height of the C19:0 internal standard [8]. 2.4. OCT imaging Images were acquired using a commercially available frequency-domain OCT imaging system (ILUMIEN; St. Jude Medical...
MbcS, methylbutyryl-CoA synthetase; PG, phosphatidylglycerol. Selectivity for short-chain acid incorporation The incorporation of unlabeled aC5 was detected as described above; however, measurements of the incorporation of iC4 and iC5 required the use of mass-tagged [d7]isobutyrate and [d9]...
Rapid preparation of fatty acid methyl esters from lipids. Lab. Pract. 22, 475–476 passim (1973). Shirai N, Suzuki H, Wada S. Direct methylation from mouse plasma and from liver and brain homogenates. Anal. Biochem. 2005;343(1):48–53. https://doi.org/10.1016/j.ab.2005.04.037. ...
Fatty acid methyl ester extracts were then re- suspended in 0.5 mL of iso-octane including C21:0 (me- thyl heneicosanoin (0.1 mg/mL)) as a standard and subsequently analyzed by gas chromatography–mass spec- trometry as described by Mietkiewska et al. [49]. A. Athaliana transformation...
Fatty acids and HFAs extraction and the corresponding methyl esters preparation To extract the FFAs and HFAs from the fermentation broth, the culture broth was acidified with 6 M hydrochloric acid to pH < 2. Eicosanoic acid (C20), 10-hydroxydecanoic acid methyl ester (10-OH-C10) or ...
SDS-PAGE analysis and chemiluminescent detection revealed that the major cytosolic proteins that bound this biotinylated probe had molecular masses of 43 and 51 kD. Fatty acid competition experiments indicated that the order of effectiveness in displacing this probe from these proteins was 13(S)-HODE...
et al. Nitrogen Deprivation Induces Lipid Droplet Accumulation and Alters Fatty Acid Metabolism in Symbiotic Dinoflagellates Isolated from Aiptasia pulchella. Sci Rep 4, 5777 (2014). https://doi.org/10.1038/srep05777 Download citation Received01 April 2014 Accepted27 June 2014 Published22 July 2014...
Formation of fatty acid ethyl esters (FAEEs, catalyzed by FAEE synthase) has been implicated in the pathogenesis of chronic pancreatitis. In previous studies, we demonstrated that FAEE synthase, purified from rat liver microsomes, is identical to rat liver carboxylesterase (pI 6.1), and structural...
Gtα transducin α-subunit FAME fatty acid methyl ester FAPE fatty acid phenacyl ester Gtβ transducin β-subunit Gtγ transducin α-subunit HPLC high pressure liquid chromatography PAGE polyacrylamide gel electrophoresis ROS rod outer segments GTPαS guanosine 5′-3-O-(thio)triphosphate. ...
Protein concentration was determined on cell lysates using a bicinchoninic acid-based assay (Thermo Fisher Scientific). Samples were then denaturated (7 min, 95°C) with Laemmli sample buffer containing 100 mM dithiothreitol. Afterwards, samples (20 μg per well) were separated by SDS-PAGE (15...