Fate‐mapping studies in inbred mice: A model for understanding macrophage development and homeostasis?doi:10.1002/eji.202250242RETICULO-endothelial systemMACROPHAGESBONE marrowEMBRYOLOGYYOLK sacCD14 antigenThe mononuclear phagocyte system (MPS) was defined in the early 1970s as a family of cell...
while Cx3cr1 + macrophages are derived from adult hematopoietic stem cells. Aging has a profound effect on the macrophages in the thymus. Timd4 + cells underwent gradual attrition, while Cx3cr1 + cells slowly accumulated with age and, in older mice, were the dominant macrophage population in...
k MDA-MB-231 hypoxia fate-mapping cells were injected into the tail vein of NSG mice and harvested 5 weeks later Full size image Three weeks following tumor removal, we assessed the ability of GFP+ cells to form overt macrometastasis. GFP+ cells were 6–7× more likely than DsRed+ ...
Thus,barcoding enables high-resolution fate mapping in essentially all tissues in mice for which inducible Cre driver lines are available. Alternative methods include ex vivo cell barcoding, inducible transposon insertion and CRISPR鈥揅as9-based barcoding;currently allows combining non-invasive and cell-...
摘要: With the new understanding that adult microglia in mice have embryonic origins and are maintained in situ throughout life, it has become pertinent to now understand how these unique cells differ from...关键词: Cx3cr1-CreER mice Flow cytometry In vivo/fate mapping Microglia Monocyte-...
Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy. Ovaries of donor FVB/N-Tg (Pol II–Luc) Ltc transgenic mice (n = 5) were cryopreserved and transplanted to the bac
As a step toward generating a fate map of identified neuron populations in the mammalian hindbrain, we assessed the contributions of individual rhombomeres to the vestibular nuclear complex, a major sensorimotor area that spans the entire rhombencephalon. Transgenic mice harboring either the lacZ or ...
In contrast to many macrophage populations, we show that microglia develop in mice that lack colony stimulating factor-1 (CSF-1) but are absent in CSF-1 receptor–deficient mice. In vivo lineage tracing studies established that adult microglia derive from primitive myeloid progenitors that arise ...
We used fate mapping and AID(GFP) reporter mice to determine if AID expression in the mouse extends beyond lymphocytes. We discovered that AID(cre) tags a small fraction of non-lymphoid cells starting at 10.5 days post conception (dpc), and that AID(GFP+) cells are detectable at dpc 11.5...
展开 关键词: Germinal Center Dendritic Cells, Follicular Lymph Nodes Stromal Cells Stem Cells Mesoderm Animals Mice, Inbred C57BL Mice, Transgenic Mice, Knockout DOI: 10.1084/jem.20132409 被引量: 83 年份: 2014 收藏 引用 批量引用 报错 分享 全部...