The location of stained cells in the mature organism was then noted. Pooled results of several similar experiments allowed construction of a diagram known as a fate map. Therefore, a fate map is an overall plan that outlines the fate of each part of an early embryo. These maps help ...
To avoid that problem, as well as technical limitations of current methods for mapping cell fate, we, and others, have developed a new strategy for retrospectively deriving cell fate maps by using phylogenetics to infer the order in which somatic mutations have arisen in the genomes of ...
We computed fate probabilities and summarized them in a fate map (Fig.2e). This analysis correctly identified the beta cell fate as dominant in the Ngn3highEP cluster at E15.5, consistent with known biology24(Fig.2e, inset), as also visualized with pie charts on a directed implementation of...
接着,作者总结了the strengths and limitations for constructingfate maps—schematics representing the developmental potential of specific cells or regions of cells within an embryo at a defined stage, as outlined at the beginning of this review. 13.1 Advances in Cell Imaging for High-Resolution, Non-...
We generated genome-wide RT maps from sorted ESCs and 2CLCs in early, mid and late S-phase (Extended Data Fig. 6a–c). A survey over the genome browser revealed specific gene regions shifting to earlier RT in 2CLCs. These included ‘2C’-specific genes such as Zscan4, Obox2/3 and...
Cell Fate Specification in Glossiphoniid Leech: Macromeres, Micromeres, and Proteloblasts D Weisblat,F Huang,DE Isaksen 被引量: 0发表: 1999年 Micromere fate maps in leech embryos: lineage-specific differences in rates of cell proliferation Stereotyped early cleavages in glossiphoniid leech ...
a Aggregated the fate maps of cell clusters in Jin668 and TM-1 into primary vascular cells using directed edges. The pie charts to show cell fates averaged per cluster. Edges between clusters are given by transcriptomic similarity between the clusters. b–d The fate maps of directional ...
Comprehensive RNA structural maps during zebrafish early embryogenesis.aSchematic view of in vivo RNA structural maps during zebrafish early development using icSHAPE.bNucleotide composition in transcriptome and all profiled sites (transcriptome, A: 27.91%, U: 25.96%, C: 22.47%, G: 23.66%; icSHAPE...
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In the present study, we have used the high-throughput ChIP-Seq approach to generate genome-wide H3K4me3 and H3K27me3 maps in naive, Th1, Th2, Th17, iTreg, and nTreg cells to gain insights into lineage commitment and plasticity in developing T cells. Our results point to surprising ...