而Lineage tracing, stemming from century-old fate mapping experiments, aims to construct a hierarchy of all progeny arising from an individual cell but does not necessarily capture positional information.同时,While stemming from fate mapping, lineage tracing is distinct in its aim to reconstruct ...
4d, e). By contrast, using the in silico fate mapping data alone did not single out the correct model (Supplementary Fig. 4f). This example with computationally simulated data suggests that lineage pathways downstream of tip HSCs can be inferred from integrating experimental data on stem cell f...
Fate-mapping experiments at early somite stages in chick have revealed that the cerebellar primordium is located both anterior and posterior to the midbrain-hindbrain constriction, whereas midbrain precursors lie more anteriorly. Fate mapping in mice has been complicated by the inaccessibility of the ...
Fate mapping withCX3CR1YFP−CreER/wt:R26RFPmice reveals that microglia and recruited mo-MFs are phenotypically distinguishable in light injury. (a) Fate mapping inCX3CR1YFP−CreER/wt:R26RFPmice differentially label microglia (i.e. YFP+RFP+) vs. blood Mo (i.e. YFP+RFP−), which ...
Fate-mapping experiments at early somite stages in chick have revealed that the cerebellar primordium is located both anterior and posterior to the midbrain-hindbrain constriction, whereas midbrain precursors lie more anteriorly. Fate mapping in mice has been complicated by the inaccessibility of the ...
Results of these fate-mapping experiments revealed that the lateral and posterior epiblast contain cells that will give rise predominantly to mesodermal derivatives. The various mesodermal populations are distributed in overlapping domains in the lateral and posterior epiblast, with the embryonic mesoderm ...
Journal of Visualized Experiments www.jove.com Video Article Single Cell Fate Mapping in Zebrafish 来自 core.ac.uk 喜欢 0 阅读量: 20 作者:V Kohli,K Rehn,S Sumanas 摘要: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits ...
The resulting double transgenic line Tg(hsp70:mCherry-T2a-CreERT2;coro1a:loxP-DsRedx-loxP-GFP) was used for microglia fate mapping analysis (Figure 1A). To achieve the temporal-spatial restricted cell labeling, we applied the infrared laser-evoked gene operator (IR-LEGO) microscope heating ...
A total of 91.3% mapping rate and more than 3000 cells for each sample was captured. All feature-barcode matrices of samples from different induced time points (0, 1, 6, and 12 HACI) for both TM-1 and Jin668 with two biological repetitions were then aggregated using “cellranger aggr”...
CellRank for directed single-cell fate mapping Abstract Computational trajectory inference enables the reconstruction of cell state dynamics from single-cell RNA sequencing experiments. However, trajectory inference requires that the direction of a biological process is known, largely limiting its application...