slides were counterstained in Hematoxylin (Bio-Optica) and images were acquired by Leica DMRD optical microscope (Leica). For immunofluorescence, immunostainings were developed using TSA Plus Cyanine 3, TSA Plus Cyanine 5, or TSA Plus
Z-DNA and Z-RNA are nucleic acids with an alternative left-handed double-helix structure and have poorly understood biological function10,11,12,13. These Z-form nucleic acids are recognized by specific protein domains, termed Zα domains, which bind Z-DNA and Z-RNA in a conformation-specific...
sds.o async.o read.o sockcompat.o cc -std=c99 -pedantic -c -O3 -fPIC -DHIREDIS_TEST_SSL -Wall -W -Wstrict-prototypes -Wwrite-strings -Wno-missing-field-initializers -g -ggdb ssl.c ar rcs libhiredis_ssl.a ssl.o make[3]: Leaving directory '/root/redis-6.0.3/deps/hiredis' MAKE...
Some parts of a program can be swapped out along with any data that’s not required for the particular set of instructions currently being executed, but the instructions plus data cannot exceed the physical memory that’s free. The QNAP TS-431+ has a 32-bit ARM CPU, so the version of ...
For denaturing immunoprecipitation, cells were lysed in a denaturing buffer (1% SDS, 5 mM EDTA, 10 mM DTT), boiled at 95 °C for 5 min, and then diluted 10 times with the 1% Triton X-100 lysis buffer before immunoprecipitation. Equal amounts of total protein lysates or immuno...
PCR was conducted by an initial denaturation at 95°C for 5 min, 40 cycles at 95°C for 30 sec; 55°C for 30 sec; 72°C for 1 min and a final extension at 72°C for 7 min using the Hotstar Taq-Plus DNA polymerase (Qiagen). Amplification of 16S rRNA gene and BAMQ#8 target ...
(at control; 4 hr. ischemia; and 4 hr. ischemia plus 2 hr. reperfusion) were determined by repeated permutation of MIAME-compliant (www.mged.org) data using Significance Analysis for Microarray (SAM). Results from the SAM analysis were visualized as hierarchical clusters inGene Traffic(www....
Blood samples were diluted twofold with PBS (pH 7.2) and 30 ml was centrifuged over a 20-ml Ficoll-Paque PLUS cushion at 400g for 30 min without braking. The mononuclear layer was collected and washed with PBS to remove platelets. Monocytes were isolated by adherence to plastic at a ...
PLUS (GE Healthcare) gradient. Blood samples were diluted twofold with PBS (pH 7.2) and 30 ml was centrifuged over a 20-ml Ficoll-Paque PLUS cushion at 400gfor 30 min without braking. The mononuclear layer was collected and washed with PBS to remove platelets. Monocytes were isolated ...
DNA from the bone marrow, spleen, thymus, or blood cells of reconstituted mice was isolated by overnight digestion with proteinase K (0.3 mg/mL) in a 50 mmol/L Tris solution (pH 8) containing sodium dodecylsulfate (SDS 0.6%), EDTA (1 mmol/L) and NaCl (100 mmol/L). DNA (30 μg...