Fat-Acid-Free-BSA网络无脂肪酸牛血清白蛋白 网络释义 1. 无脂肪酸牛血清白蛋白 无脂肪酸牛血清白蛋白(Fat Acid Free BSA)产品及应用 | 生命 ...牛血清白蛋白(无脂肪酸),或者称为Fat Acid Free BSA,产品不 …www.gkcd.cn|基于1 个网页© 2025 Microsoft 隐私声明和 Cookie 法律声明 广告 帮助 反馈...
产品名称:Albumin, from Bovine Serum, Fatty Acid Free 无脂肪酸牛血清白蛋白订购此产品 供应商:Wako 规格:5G 目录价:790 相关信息: 无脂肪酸牛血清白蛋白 中文名: 无脂肪酸牛血清白蛋白 英文名:Albumin, from Bovine Serum, Fatty Acid Free 描述:牛血清白蛋白(Bovine serumalbumin, BSA)是常用的试剂。在血...
(BSA)—essentially fatty acid-free (Sigma-Aldrich, A6003), filtered through 0.22 μm] at 37 °C for 30–50 min with slight agitation or until homogeneous. The homogenate was then filtered through a 250 μm filter and collected in a sterile falcon tube. After a rest (~5 ...
The fat tissue was then cut into small pieces (4 mm3), pooled per genotype and placed into Krebs buffer (pH 7.4) containing 0.5% (w/v) fatty acid-free BSA, penicillin/streptomycin (1:100) and fungizone (1:100) (Invitrogen). A total of 200 mg adipose tissue was rinsed in PBS...
For all of the treatments, the the fetal bovine serum in the culture solution was replaced with fatty acid-free BSA (1 g/L), and the cells were treated with different ratios of oleic, linoleic, and linolenic acids (0.75:4:1, 1.5:10:1, 2:13.3:1, 3:20:1, and 4:26.7:1) for ...
A bicinchoninic acid (BCA) protein assay kit (P0012; Beyotime, Shanghai, China) was used to determine protein concentrations. The proteins were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to a polyvinylidene fluoride membrane. The membranes were blocked ...
To promote LD accumulation, cells were incubated with growth media supplemented with 120 µM oleate and 120 µM palmitate that had been bound to 1% fatty acid-free BSA for 24 h (17). LDs were stained with 20 nM bodipy 581/591 undecanoic acid (D3861; Invitrogen). DNA transfection HEK...
In parallel, 2% fatty-acid-free BSA was solubilized in sterile nanopure water at 37°C. The palmitic acid/BSA complex solution was then prepared by incubating the components in a molar ratio of 5:1 at 37°C in a shaking water bath for 1 h and was used directly for INS-1 stimulation ...
A total of 200 mg adipose tissue was rinsed in PBS and incubated in 100-mm Petri dishes containing 10 ml aMEM (Invitrogen) supplemented with 0.5% (w/v) fatty acid-free BSA, penicillin/streptomycin (1:100) and fungizone (1:100). All conditions were repeated in six different dishes. ...
To establish an in vitro model of hepatic steatosis, L02 cells were stimulated with 1 mM FFA containing sodium oleate and sodium palmitate (2:1 at molar ratio) and 1% fatty acid-free bovine serum albumin (BSA) for 24 h. The L02 cells used in our research has been published [47]....