fastMNN简介 MNN是Haghverdi等人提出的一种批次校正算法,由R语言的batchelor包实现。算法原理及性能评测的细节可参阅2018发表在Nature Biotechnology上的论文:Batch effects in single-cell RNA-sequencing data are corrected by matching mutual nearest neighbors. 此算法是Seurat3锚点整合算法的核心部分,也被Monocle3采纳...
During our analysis, when attempting to merge epithelial cells from different samples, their considerable heterogeneity results in a violation of the first assumption, leading to unsatisfactory UMAP plots. Indeed, the concept of Harmony is intriguing, but its implementation is notably complex, requiring ...
2#https://satijalab.org/seurat/articles/install_v5.html#2在seurat_v5文件夹下安装v5---.libPaths(c('/home/rootyll/seurat_v5/',"/usr/local/lib/R/site-library","/usr/lib/R/site-library","/usr/lib/R/library"))library(Seurat)pbmc=readRDS('~/gzh/pbmc3k_final.rds')DimPlot(pbmc)...