Fifty-one of 75 patients fasted before the PET scan, and 27 of 75 patients were studied under high-dose corticosteroid medication. Eighteen tumors had recurred. PET results were compared to histopathological grading. PET correctly identified 8/9 atypical or malignant meningiomas and 58/66 grade 1...
All patients had previously undergone a clinically indicated 18F-DCFPyL PET/CT scan [14]. A second scan was performed within one month with gustatory stimulation achieved by intake of saliva-inducing food items rich in sugar, acid, and fats shortly before and up to 10 min after tracer ...
Final blood BHB concentrations of mice that were fasted, fed a ketogenic diet, or injected with ketone bodies were recorded just before sacrifice between 10:00 a.m. and 11:00 a.m. using the FDA approved Keto Mojo blood glucose and ketone monitoring kit. This kit uses an enzymatic BHB ...
Fasting before or after wound injury accelerates wound healing through the activation of pro-angiogenic SMOC1 and SCG2. Theranostics. 2020;10:3779–92. Article PubMed PubMed Central CAS Google Scholar Apte RS, Chen DS, Ferrara N. VEGF in signaling and disease: beyond discovery and ...
TTA was dissolved in 1 M NaOH before binding to BSA. FA/BSA stock solutions were sterile-filtered through 0.22 μm μStar LB filters (Costar; #8110) and stored at −20°C for no longer than 3 weeks before use. Culturing, transfection, and lipid staining of cells COS-1 cells (...
(Supplementary Fig.1a). As reported before (e.g. ref.28), this protocol robustly elicited progressive weight loss (Fig.1a), culminating in significantly reduced body weight and adiposity at the end of the experiment (Fig.1b, c), when mice were still in the weight loss phase. Furthermore...
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Cells were washed three times with ice-cold PBS before cellular lipids were extracted with hexane/isopropanol (3:2, vol). The incorporated radioactivity in the organic phase was determined by liquid scintillation counting. Counted values were corrected by protein content. Western blot analysis Western...
The mice were fasted for 6 h before the tests. The insulin (0.75 U kg−1, Sigma Aldrich, USA) was injected, and blood glucose was measured before (0 min) and after the injection (15, 30, 60, 120 min) using a OneTouch® SelectSimple™ glucometer (LifeScan Inc., China...