fastsite-directedmutagenesiskit快速定点突变试剂盒.pdf,版本号快速定点突变试剂盒目录号产品内容产品组成储存条件收到本产品后请立即将置于条件下保存试剂盒其他组分置于条件下保存感受态细胞条件下保质期个月试剂盒其他组分在条件下保质期年本产品仅供科研使用请勿用于医
10912ES50*Hieff CloneTMPlus Multi One Step Cloning Kit多片段一步法快速克隆试剂盒 11001ES76*Dpn I(甲基化模板消化酶) 11003ES10*Hieff MutTMSite-Directed Mutagenesis Kit定点突变试剂盒 11004ES10*Hieff MutTMMulti Site-Directed Mutagenesis Kit多点突变试剂盒 ...
Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelityTransStart®FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to...
Genomic DNA from cells or tissue was isolated using the DNeasy blood and Tissue kit (Qiagen) according to the manufacturer’s protocol. DNA was amplified using standard PCR using LongAmp Taq DNA polymerase (NEB) targeting the sgPKCα-1 editing site with a 200–500 bp-long amplicon. Used ...
The wedge mutant (79PYIP82→GGGG) was generated using the Quik-Change Site Directed Mutagenesis kit (Stratagene) and performed according to the manufacturer’s protocol. The four glycine residues were simultaneously introduced into the full-length D43A EndoV sequence by using a complementary primer...
In the biofuel industry, cellulase plays an indispensable role in hydrolyzing cellulose into fermentable glucose. Trichoderma reesei is a popular filamentous fungus with prominent ability to produce cellulase. While classical mutagenesis and modern multi
Using this kit (with gel purification of digested vectors and PCR-amplified inserts), the successful rate in our laboratory still varies significantly with overall successful rate less than 50%. In the PCR-based QuickChange mutagenesis, the number of PCR cycles is recommended to be 12-18. ...
(Origene clone, RR210027) using Quickchange XL II Site Directed Mutagenesis kit (Agilent Technologies). The open reading frame for Navβ4 protein (NP_001008880.1) betweenApaI andEcoRI was cut and inserted into mVenus N1 or pmTurquoise2 N1. Vectors, mVenus N1 and pmTurquoise2 N1, were ...
Point mutations P502A, S522A, S522D, R525E, and P526A were introduced in full length mouse CRMP4 and S774A and S778A were introduced in full length rat Dynamin-1 by site-directed mutagenesis and verified by sequencing. The list of primers used in this study is provided in Supplementary...
site-directed mutagenesis (using e.g., a kit, such as kit such as QuikChange® available from Agilent Technologies, Inc., Santa Clara, Calif.) of encoding nucleic acid molecules, or by solid phase polypeptide synthesis methods. In addition, modified chimeric proteins (i.e. Gla domain swap...