Fast Mutagenesis System目录号: FM111-01 单价:￥720规格:10 rxns 20 rxns数量:- +说明书 质检报告 立即下单 加入购物车产品详情介绍本产品以甲基化的质粒为模板,采用部分重叠引物 (均含突变点) 设计,使用2×TransStart® FastPfu Fly PCR SuperMix扩增,扩增产物用DMT限制性内切酶消化甲基化质粒模板后,转化...
Fast FastMultiSite Mutagenesis System 目录号规格单价 FM201-0110 rxns1800 产品详情介绍 本产品通过在重叠区域引入突变位点的方法,利用TransStart®FastPfuFly PCR SuperMix扩增,制备包含突变的PCR片段;利用特殊的重组酶和同源重组原理,将不多于6个片段无缝拼接,从而实现多点突变。
Fast Mutagenesis System. Primers anneal to the DNA template, mutant strands are synthesized with FastPfu DNA Polymerase. In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parent
TransGenBiotech.transgenOrderLine:010-51296890CustomerService:010-83011923E-mail:trans@transgenFastMutagenesisSystem目录号:FM111保存:DMTChemicallyCompetentCell-70℃保存六个月,其它-20℃保存一年。试剂盒组成注意:质粒大小大于4kb,dNTPs使用量为2µl。PCR循环94℃2-5min94℃30sec55℃30sec20-25cycles72℃15-...
Fast Mutagenesis System
Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelityTransStart®FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to...
Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast...
Traditionally, forward and reverse genetics using targeted mutagenesis in combination with transgenesis has been used. More recently, clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genome editing has become the method of choice for gene engineering in many species and tissues1....
In the biofuel industry, cellulase plays an indispensable role in hydrolyzing cellulose into fermentable glucose. Trichoderma reesei is a popular filamentous fungus with prominent ability to produce cellulase. While classical mutagenesis and modern multi
5). By contrast, in control cortical axons, mitochondrial inhibition has a negligible impact on sv-ATP levels, potentially compensated by glycolysis-derived ATP in conjunction with the creatine kinase system [123]. These observations suggest that NMNAT2 loss results in a shift towards mitochondrial ...