Total internal reflection fluorescence (TIRF) microscopy offers powerful means to uncover the functional organization of proteins in the plasma membrane with very high spatial and temporal resolution. Traditional TIRF illumination, however, shows a Gaussian intensity profile, which is typically deteriorated ...
All options for post-processing were disabled, which resulted in weak filtering of the velocity vectors. The direction of the velocity vectors was set relative to the centre of the nucleus. The centre of the nucleus was determined on the basis of the centre of mass of the widened smoothing ...
In the last twenty years, many restoration methods have been investigated in order to “inverse” the model in 2D-3D fluorescence microscopy. The most popular restoration approaches are linear methods (e.g., Wiener filtering). Despite the simplicity and low-computation-requirement, they usually do...
of probes, each probe representing a relative position at which at least one test is performed in an image at a given pose, each such test contributing evidence that the pattern exists at the pose; providing the run-time image; comparing the model with the run-time image at each of a ...
We present the first super-resolution images of cellulose bundles in the plant cell wall produced by direct stochastic optical reconstruction microscopy (dSTORM) in combination with total internal reflection fluorescence (TIRF) microscopy. Since TIRF limits observation to the cell surface, we tested as...
interleaved square patches, clustering those patches based on similarity, and then filtering them before reconstructing the image. BM3D, however, is not blind and takes, as a parameter, an estimate of the standard deviation of the underlying noise. Moreover, BM3D does not work on Poisson noise....
Total internal reflection fluorescence (TIRF) microscopy offers powerful means to uncover the functional organization of proteins in the plasma membrane with very high spatial and temporal resolution. Traditional TIRF illumination, however, shows a Gaussian intensity profile, which is typically deteriorated ...
Firstly, we performed mean filtering on the original image to obtain a smoothed reference image 𝐼0I0, as shown in Figure 12a. Figure 12. Blind hole region extraction. (a) Reference image 𝐼0I0. (b) Local threshold segmentation. (c) Region filling and connected component segmentation. ...
The reflecting power of an interface is governed by the density contrast of the materials on both sides. Often these densities are expressed as refraction indices. While optical light probes the polarizability and X-rays the electron density, the here-used neutrons interact with the atomic nuclei,...
A cutoff beamsplitter (Model: HB720, Zhenhua Optoelectronics, Nantong, China) with 50% transmission and 50% reflection was used to separate the excitation light and fluorescence, and a filter was added between the beamsplitter and the circuit for filtering treatment. The cross-section diagram of...