Game and movie studios are switching to physically based rendering en masse, but physically accurate filter convolution is difficult to do quickly enough to update reflection probes in real-time. Cubemap filtering has also become a bottleneck in the content processing pipeline. We have developed a ...
1e) that are similar to those of conventional 3D-SIM: spectrum separation, parameter estimation, spectrum shifting and Wiener filtering. A remarkable distinction, however, was the handling of the orders ±1 spectra, which should be further divided into upper and lower parts (Fig. 1e and ...
All options for post-processing were disabled, which resulted in weak filtering of the velocity vectors. The direction of the velocity vectors was set relative to the centre of the nucleus. The centre of the nucleus was determined on the basis of the centre of mass of the widened smoothing ...
Total internal reflection fluorescence (TIRF) microscopy offers powerful means to uncover the functional organization of proteins in the plasma membrane with very high spatial and temporal resolution. Traditional TIRF illumination, however, shows a Gaussian intensity profile, which is typically deteriorated ...
“Scattering” boundary conditions were used at the outer boundaries to prevent the reflection of the wave at the boundary, and “Matched” boundary conditions were used at the top and bottom to launch the Gaussian beam at 633 nm with a waist beam waist of 5 μm to the simulation domain....
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We present the first super-resolution images of cellulose bundles in the plant cell wall produced by direct stochastic optical reconstruction microscopy (dSTORM) in combination with total internal reflection fluorescence (TIRF) microscopy. Since TIRF limits observation to the cell surface, we tested as...
Total internal reflection fluorescence (TIRF) microscopy offers powerful means to uncover the functional organization of proteins in the plasma membrane with very high spatial and temporal resolution. Traditional TIRF illumination, however, shows a Gaussian intensity profile, which is typically deteriorated ...
The most popular restoration approaches are linear methods (e.g., Wiener filtering). Despite the simplicity and low-computation-requirement, they usually do not restore fine image details at frequency components that are beyond the bandwidth of the PSF (i.e., the support of its Fourier ...
Measurements may be performed over broad spectral bands all the way down to monochromatic ranges, where it is necessary for each of the outlet focusing means for the separated radiation to be preceded by interference filtering means. The focusing means preferably include achromatic doublets. ...