Log-log graph paper or computer and software for ELISA data analysis Protocol Outline Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. ...
Protein palmitoylation was determined with an adapted acyl-biotinyl exchange protocol from Drisdel and Green,17 which is comprised of the following three steps. Cell lysates were prepared in 250 mM HEPES, 1 mM EDTA and 2.5% SDS, and incubated with 0.1% methyl methanethiosulfonate (Sigma, St ...
(b) WSU cells stably transfected with constructs encoding forhFasL,hFasLC82SorhFasLA247Ewere subjected to an acyl-biotinyl exchange protocol as described in Materials and Methods. Specificity of the experiment was controlled by omitting the hydroxylamine (HA) treatment and Fyn was used as an ...
FITC-conjugated anti-rat CD4, PE-conjugated anti-rat CD25, and PE-Cy5-conjugated anti-mouse/rat Foxp3 antibodies (all from eBioscience) were used for staining Treg cells according to the protocol recommended by eBioscience. For intracellular cytokines analysis, MNC were fixed with 2 % parafor...
Cell death in FFPE lung tissue was detected resourcing to a TUNEL Assay kit - ApopTag® Red In situ Apoptosis Detection Kit (Millipore, Billerica, MA, USA, S7165). Staining was performed according to the manufacturer protocol. Briefly, sections were deparaffinised and hydrated followed by ant...
volunteer before inclusion in the study and the endoscopic inspectors were blinded toward the groups before the endoscopic examinations. The study protocol and the acquisition of the tissue samples were approved by the Institutional Review Board of The Third Affiliated Hospital of Sun Yat-Sen ...
(all from eBioscience) were used for staining Treg cells according to the protocol recommended by eBioscience. For intracellular cytokines analysis, MNC were fixed with 2 % paraformadehyde for 20 min at 4 °C and permeabilized with 0.5 % saponin. Then, the cells were stained with PE-...
Treatment of Jurkat cells with hHis6FasL induces casapase-3 cleavage as detected by PathScan® Cleaved Caspase-3 (Asp175) Sandwich ELISA Kit #7190. Orders: 877-616-CELL (2355) • orders@cellsignal.com • Support: 877-678-TECH (8324) • info@cellsignal.com •...