and the muscular tissues were collected for qRT-PCR and Western blot analysis. For the AAV-mediated FISC system experiment, theGt(ROSA)26Sortm14(CAG-tdTomato)Hzemice (male, 8-week-old) were randomly divided into two groups with three mice in each group. These mice were transducted with a...
FLAG and integrin β5 were detected by western blotting. * indicate immunoglobulin heavy chain. c Correlation between FARP1 mRNA expression and integrin β5 mRNA expression in primary gastric cancer based on TCGA data. d Transwell migration assay in MKN74 cells transfected with NC Si, FARP1 Si...
Expressions of the respective PolyQ proteins with a mass of 67 KDa and 73 KDa in the MJD26 and MJD78 cells were confirmed by western blotting (Fig. 1a,b). Both of MJD26 and MJD78 expressed dramatic increase of endogenous ataxin-3 compared to the wild type (WT) because the late-...
To explore the expression of FBP1 in NPC cells and tissues, we first analyzed the levels of the FBP1 protein and mRNA in two non-cancerous primary human nasopharyngeal epithelial cells and 12 NPC cell lines by western blot and quantitative real-time PCR (qRT-PCR). As shown inFigures 1a ...