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After graduating with Magna Cum Laude honors, she attended Midwestern University College of Dental Medicine, in Downers Grove, Illinois, where she received her Doctorate of Dental Medicine. Following dental school, she moved to St. Louis, Missouri to complete her hospital dentistry residency at ...
Egg white must provide nutrients and protection to the developing avian embryo. One way in which this is achieved is an arsenal of antimicrobial proteins and peptides which are essentially extensions of the innate immune system. Gallin is a recently iden
After incubation with HRP-conjugated secondary antibodies (1:5000, goat anti-rabbit or mouse, Cell Signaling), specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagents according to the manufacturer's instructions (Pierce, Rockford, IL) and then recorded with ...
For Western blots, whole-cell protein extracts were prepared from peripheral blood mononuclear cells (PBMCs) by using radio immunoprecipitation assay (RIPA) lysis buffer (Thermo Scientific, Rockford, USA). Protein concentrations were determined using the BCA Protein Assay Kit (Pierce, Thermo Scientific...
, Current Protocols in Human Genetics (John Wiley & Sons 1998), and Richards and Ward, “Molecular Diagnostic Testing,” in Principles of Molecular Medicine, pages 83-88 (Humana Press, Inc. 1998)). Direct analysis of an zcyto20 gene for a mutation can be performed using a subject's ...
Lysates were resolved by SDS-PAGE, and Western blotting was carried out using horseradish peroxidase-conjugated IgG as a secondary antibody and the ECL system (Thermo Scientific, Rockford, IL, http://www.piercenet.com) for detection. Analysis of mitochondria density For density quantification, ...
Growth hormone receptor (GHR) plays a vital role in breast cancer chemoresistance and metastasis but the mechanism is not fully understood. We determined if GHR could be a potential therapeutic target for estrogen receptor negative (ER−ve) breast canc