First Medical Associates’ Primary Care Centers are dynamic, modern practices delivering high-quality, individualized primary care in multiple convenient locations in Rockville, Germantown, Gaithersburg, Columbia, Bowie, Nottingham, Frederick, Severna Park, and Silver Spring, Maryland. We provide a compreh...
The PCR fragment was amplified with these primers (Forward aagaaggagatatacatatgAAATCGAAAATGGATCCAGAAG; Rerverse gcggtcggcagcaggtattttcagtggtggtggtggtggtgTGAATTGCTGGCAGTTGAC) and purified with QIAquick PCR purification kit (Qiagen, Germantown, MD USA), before cloning with SLIC cloning method ...