出现集数:S2E16/Stewie Grffin the Untold Storypeter因向黑帮借高利贷买新车还不起,被迫和big fat paulie去看电影。死因:在说出密码之前被强手给drive by了。 imperviious stewie 7 4. Bitch Brian and Bitch Stewie出现集数:S8E06Stewie为了减轻负担为自己克隆了一个智商是硬伤的克隆人Bitch Stewie.后来也为...
[1]回看Succession前三季,Jesse及其编剧室笔下的E3尽管不乏一些让人印象深刻的场景(S1E3末尾Greg偶遇公园长椅上密谈的Sandy和Stewy;S2E3里Logan命令众人玩“Boar on the floor”;S3E3中Kendall在电视台长廊独行),但这些场景通常只在单集内部具有牵拉叙事张力和打开角色生成空间的动能。 将Logan离世的情节安排在S4E3,...
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Shanker (Scientific Editing, St. Jude) for editing this manuscript; Richard Cross, Greig Lennon, and Parker Ingle (Microfluorimetry, St. Jude) for cell sorting; Ashutosh Mishra and Vishwajeeth Pagala (Proteomics, St. Jude) for LC-MS/MS analysis; Cliff Guy (Confocal Imaging, St. Jude) for...
The MoARF and MoGGA1 genes with their corresponding native promoters were cloned into both pXY203 (S tag) vector and pYF11 (GFP tag) vector with primers (Table S2). After sequencing, construct pairs were transformed into the protoplasts of Guy11. The total proteins were extracted from trans...
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(D) Radioactive 3H-riboflavin uptake assays using appropriate E coli cells (strain LMG194 derivatives, SI Table S2) expressing either CcoA or RibN or the CalT-O, -R or -A. pBAD corresponds to the same cells carrying an empty expression vector (SI Table S2). In each case, the...
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As MMTV-PyVT transgenic mice are known to develop multifocal mammary tumors that spontaneously metastasize to the lung (Guy et al., 1992a), we first crossed these mice with our miR-22F/+;MMTV-Cre mice and analyzed the resultant compound mutants for development of primary tumors and lung ...
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