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To that end, we have transfected SH-SY5Y cells with Flag-tagged Pea3 expression vector, and immunoprecipitated using Flag antibody. When precipitated DNA was analyzed with qPCR, Pea3 was found to bind efna3 promoter, which was found to be upregulated by all three Pea3 family members, but ...
An empty vector lacking ANKRD11 sequence was used as a negative control. The integrity of ANKRD11 sequence within the plasmid constructs was confirmed via Sanger sequencing. 2.6. Cell culture and transfection Human embryonic kidney (HEK293) cells were obtained from the Cell Bank at the Chinese ...
For pHluorin2 signal calibration, pHluorin2 was fused with MBP on pMal vector, and MBP-pHluorin2 proteins were expressed in BL21 E. coli strain. Harvested bacterial cell pellets were homogenized by sonication in lysis buffer (50 mM Tris-HCl, pH 8.0, 300 mM NaCl, 1 mM EDTA) ...
Both JJN3 and RPMI-8226 were infected by empty lentiviral vector, used as control, or ING4 overexpression vector, as described in “Cells and cell culture conditions; siRNA transfection.” p29ING4 protein was detected after 72 hours by Western blot analysis (F). RPMI-8226 cells overexpressing...
You probably need a vector t-shirt template, don’t you. “Uppercase Magazine is a new quarterly publication for the ‘creative and curious.’” Looks lovely. (Preview) Need to enlarge rasterized images? Photoshop certainly doesn’t cut it, but Scott Hansen reviews Genuine Fractals, a ...
After washing, cells were incubated with appropriate secondary antibodies conjugated with Alexa-488 for 1 h at RT with Hoechst 33258 and fluorescent images were taken on an Olympus OX-71 equipped with a CCD camera. Establishment of Hae ES cells The Drosophila SoxN knock-in vector was generated...
To study the function of theScJAZ6gene in response to pathogen infection, the overexpression vector pCAMBIA 1301-ScJAZ6was constructed (the primers used are listed in Table2). The recombinant vector and the control were separately transformed intoAgrobacteriumstrain EHA105 cells, and then cultured...
Undifferentiated EB5 mouse embryonic stem cells, a subline generated by targeted integration of an Oct3/4-IRES-BSD-pA vector into the Oct3/4 allele and carries the blasticidin S-resistant selection marker gene driven by the Oct3/4 promoter, were maintained on gelatin-coated dishes in Glasgow ...
To generate YAP-knockdown MDCK cells, MDCK cells were infected with lentivirus containing shRNA-targeting YAP (pLKO1-shYAP1, Addgene, 27368) or Human Mission TRC1 sequence-verified YAP shRNA lentiviral plasmid vectors (TRCN0000107265, Sigma-Aldrich) and control plasmids, pLKO1 empty vector (Sig...