长链非编码RNA FAM83H-AS1是一种新LncRNA,转录自邻近FAM83H基因对侧的8q24.3染色体.有阶段性研究发现FAM83H-AS1在许多癌症中表达水平都升高,其中包括结直肠癌,肺癌,胃癌,膀胱癌,宫颈癌,但其在肿瘤中发挥作用的分子机制尚不明确.为明确LncRNA FAM83H-AS1在乳腺癌细胞发生发展过程中的主要作用,初步查究其在肿瘤中...
The interactions between FAM83H-AS1, CDO1, and DNA methyltransferase1 (DNMT1) were analyzed by RNA-binding protein immunoprecipitation or chromatin immunoprecipitation assay. The xenograft mouse model was utilized to test CDO1 and FAM83H-AS1's influence on tumor development in vivo. Results showed ...
结论FAM83H-AS1基因在乳腺癌中高表达,与患者的不良预后、临床病理分期、肿瘤微环境及TMB均有相关性。同时FAM83H-AS1与免疫检测点相关的一些基因及错配修复基因存在相关性,以上可能为临床乳腺癌的治疗、预测预后及基因靶向药物的研制提供理论依据。 【总页数】7页(P1-6) 【作者】李娜;袁军;张安志;胡楚玲;徐茂义 ...
基金 河南省科技攻关项目(222102310488) 河南省医学科技攻关联合共建项目(LHGJ20220794)。 关键词 lncRNA FAM83H-AS1 侵袭 子宫内膜癌 miR-485-5p 分类号 R737.33 [医药卫生—肿瘤] 登录即可查看详情 中国妇产科临床杂志 2024年 第5期 职称评审材料打包下载 ;...
Long non-coding RNAs (lncRNAs) play an important role in gene regulation and are emerging as major players in cancer biology, This study investigates the expression of FAM83H-AS1 in breast cancer and its association with tumor grade, hormone receptors, pathological diagnosis, and molecular markers...
在肺腺癌中,FAM83H mRNA高表达组与低表达组生存曲线比较,差异有统计学意义(P<0.05)。A549和SKOV3细胞中,siFAM83H组FAM83H mRNA及蛋白表达低于siNC组(P<0.05);siFAM83H-AS1组FAM83H-AS1 mRNA表达低于siNC组(P<0.05);siFAM83H组转染24、48、72 h后细胞增殖能力低于siNC组(P<0.05);siFAM83H-AS1组转染24、...
The long noncoding RNA FAM83H-antisense RNA 1 (FAM83H-AS1) is involved in gastric cancer (GC) development. This study determined whether FAM83H-AS1 was regulated by N6-methyladenosine (m6A) modifications in GC. Real-time quantitative polymerase chain reaction was performed to determine the expres...
与FAM83H-AS1,HOXC10的靶向关系.结果胶质瘤组织中FAM83H-AS1表达水平显著升高,miR-136-5p表达水平降低(P<0.05);沉默FAM83H-AS1表达或过表达miR-136-5p,U87细胞凋亡率,Bax蛋白表达显著升高,细胞存活率,HOXC10 mRNA,Cyclin D1,Bcl-2蛋白表达显著降低(P<0.05);双荧光素酶报告基因显示,miR-136-5p与FAM83H-AS1...
FAM83H-AS1Gastric cancerLong non-coding RNATo explore the expression level and to investigate the clinical associations of the long non-coding RNA (lncRNA) FAM83H-AS1 in gastric cancer. The expression level of FAM83H-AS1 were explored by quantitative reverse transcription PCR (qRT-PCR). The ...
与sh-NC组比较,sh-lnc RNA FAM83H-AS1组细胞增殖和侵袭减少(P<0.05).lnc RNA FAM83H-AS1过表达对子宫内膜细胞增殖和侵袭有促进作用,而mi R-485-5p能够逆转这种促进作用.PAK1基因是mi R-485-5p的靶基因,且与lnc RNA FAM83H-AS1正相关.结论m6A修饰的lnc RNA FAM83H-AS1竞争性吸附mi R-485-5p抑制其表达...