Cas14a核酸酶是一种内切核酸酶,其在tracrRNA:crRNA(或sgRNA)的引导下,特异结合并切割靶标ssDNA,且无需PAM位点。相比于其他的Cas蛋白,Cas14蛋白的分子量普遍较小(400-700aa);与Cas12类似,Cas14a也可以结合靶标核酸并激活其ssDNA反式切割活性,从而被应用于靶标核酸的分子检测;与Cas12不同的是,Cas14a只能结合...
Cas12a的靶基因是ssDNA和dsDNA,并且Cas12a引导链需要识别PAM序列(Cas12a的PAM序列为TTTV)。Cas12a 核酸酶(其他名称 Cpf1)是依赖于 RNA 介导的内切核酸酶,在靶标 DNA 存在 PAM 的情况下, 可特异地切割靶标双链 DNA。与 Cas9 不同:(1)Cas12a 只需要单个 crRNA,且体积更小、更易 被递送至细胞中;(2)Cas...
DBCO-PEG:VHH-azide 二苯并环辛炔修饰聚乙二醇-叠氮化物 DBCO-ssDNA-biotin 二苯并环辛炔(DBCO)-生物素端修饰的单链DNA NaYF4;Yb,Er@SiO2-dsDNA-biotin 生物素修饰二氧化硅修饰单链DNA-稀土上转换 NaYF4;Yb,Er@SiO2-ssDNA-N3 叠氮修饰二氧化硅修饰单链DNA-稀土上转换 (FA-PEG)69-MS2-(DOTA-Eu)965 叶酸-...
Fig. 2: FAM111A binds to ssDNA through its central region. a Electrophoretic mobility shift assay (EMSA). DNA substrates labeled with IRDye700 (ss: single-stranded, ds: double-stranded, Y Y fork, dsY double-stranded Y fork) were incubated with increasing amounts of recombinant GST-FAM111...
(clone RAM34, cat.#560230, BD Biosciences, 1/50), and Streptavidin APC-Cy7 (cat.#554063, BD Biosciences, 1/150). Magnetic depletion of biotin-positive cells was performed before fractionating the (CD45−CD11b−CD31−Sca1−) α7-integrin+CD34+population by flow cytometry (BD FACS...