Yes, it is possible. The amplicons do not have to be the same length in order to duplex the assays. You need to use different reporter dyes on the probes in multiplex - they should have a substantial difference in maximum emission wavelength to be effective. A classic example is to use ...
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To probe the functional significance of our structural observations, we characterized the ability of FAM105A to cleave the eight different Ub chain types. Consistent with a deficiency in the catalytic triad, FAM105A was not able to cleave any of the diUb chain substrates. In contrast, OTULIN ...
Secretion of famsin was abrogated by inhibition of proprotein convertases (Fig.1e). Overexpression of furin, but not other proprotein convertases, dramatically enhanced the level of famsin (Fig.1f). In fact, furin can cleave wild-type (WT) Gm11437 but not a mutant of Gm11437 (K190A/R19...
we simulated the active protein-mediated curvature induction process. Bilayer patch-closure simulations have been used previously to study elastic properties of the membrane24,25. Here, we used the bicelle system to probe the active curvature induction capacity of an embedded protein inclusion. Using ...