产品名称:CRISPR核酸检测试纸条(Biotin/FAM) 英文名称:CRISPR SHERLOCK CRISPR SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) is a nucleic acid detection technology that utilizes CRI…
CRISPR条是一种基于CRISPR技术的快速检测工具,用于识别特定的核酸序列。通过结合生物素(Biotin)和荧光标记(FAM)的技术,这种试纸条可以实现高灵敏度和高特异性的检测。CRISPR SHERLOCK(Specific High-sensitivity Enzymatic Reporter unLOCKing)是其中一种应用广泛的CRISPR核酸检测技术,能够高效地检测和识别特定的DNA或RNA序列。
a process which is partly controlled by intestine-derived hormones. However, it is unclear whether the intestine plays an important role in metabolism during fasting. Here we have identified a novel hormone, famsin, which is secreted from the intestine and promotes metabolic...
Finally, to further validate that Stat3 and MyoD regulateFam3aexpression, we performed reporter assays using a construct containing the luciferase reporter gene under the control of theFam3apromoter. HEK293 cells were transiently transfected with the reporter plasmid and a Renilla encoding plasmid (to ...
Biotin-Streptavidin Affinity Purification Cell pellets were resuspended in 10 mL of lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 0.1% SDS, 1:500 protease inhibitor cocktail (Sigma-Aldrich), 1:1,000 turbonuclease), incubated on an end-ove...
circFAM73A sequence but had no effects on the reporter containing circFAM73A with the mutant miR-490-3p-binding site in BGC823 and SGC7901 cells (Fig.4c). Furthermore, compared with the mutant biotin-labeled miR-490-3p, wild-type miR-490-3p captured more circFAM73A in GC cells with...