FAdV-4FAdV-8b病毒分离鉴定包涵体肝炎肝炎-心包积液综合征二价疫苗禽腺病毒(Fowl aviadenovirus,FAdV)属于腺病毒科腺病毒属.主要引起的疾病包括包涵体肝炎(Inclusion body hepatitis,IBH),肝炎-心包积液综合征(Hepatitis-hydropericardium syndrome,HHS)和肌胃糜烂(Gizzard erosions,GE).禽腺病毒4型(FAdV-4)和禽腺...
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本发明公开一种用于检测并鉴别FAdV4和FAdV8b的特异性引物及其应用,属于生物技术领域.本发明根据FAdV4和FAdV8b的保守序列设计了两对特异性引物,扩增的片段大小分别为138bp,122bp,解链温度分别为81.5℃,87.3℃,可根据荧光定量扩增曲线及溶解曲线对FAdV4和FAdV8b进行区分.特异性实验表明,本发明对其它家禽常见病毒AIV(...
These results demonstrated that fiber of FAdV-8b could replace the role of fiber-1 of FAdV-4 in the process of viral infection, and rFAdV-4-fiber/8b could be used to make a potential bivalent vaccine for the control and prevention of HHS and IBH....
The presence of viral antigens in the liver, lungs, and intestine was confirmed by immunohistochemistry. To our knowledge, this is the first report of the characterization of an outbreak of inclusion body hepatitis in turkeys (hybrid converter breeds) due to FAdV-8b in Egypt. This finding ...
This study is the first report of the isolation and identification of FAdV serotypes associated with IBH in commercial broilers and broiler-breeder flocks in Turkey. The results of sequence analysis showed that FAdV-8b and FAdV-11 were the circulating serotypes that caused recent field outbreaks ...
The optimal primer concentration combination was set at 0.125 渭M for FAdV-4, -8b, and 0.25 渭M for FAdV-11. Under these conditions, the limit of detection (LOD) was 103 copies/渭L of plasmid standards for FAdV-4, -8b, and -11. These results demonstrated that the developed multiplex ...
The optimal primer concentration combination was set at 0.125 渭M for FAdV-4, -8b, and 0.25 渭M for FAdV-11. Under these conditions, the limit of detection (LOD) was 103 copies/渭L of plasmid standards for FAdV-4, -8b, and -11. These results demonstrated that the developed multiplex ...
PCR analysis confirmed the presence of FAdV, belonging to species E, serotype FAdV-8b. Performance and financial calculations revealed that IBH increased Feed Conversion Ratio (FCR), feed cost/chick as well as feed cost/kg live weight, whereas the Livability (%) and the European Production ...