(generously provided by Daniel Durocher, University of Toronto) as well as three individual single-cell HMCES-/- clones of HMCES knockouts (Clone3.1, Clone 3.3, and Clone 3.4) were seeded into 10 cm dishes and carried continuously in the presence or absence of 250 µM KBrO3 for ...
However, when cells were plated in dishes in which the same cell line had been grown to confluence and removed by scraping, logarithmic growth began immediately. The effect was not seen on areas of the plate that had been covered with coverslips or in dishes where fibroblasts had been ...
Mean colony count of duplicate dishes. There was no colony formation by either bone marrow aspirate in the absence of the colony-stimulating preparations. Cell morphology was examined by picking individual colonies by Pasteur pipette, placing them on a microscope slide, air-drying, and staining with...
InP. chrysogenum, penicillin biosynthesis and conidiation are stimulated by the addition of H2O2to the culture medium, and this effect is mediated by the bZIP transcription factors PcYap1 and PcRsmA. Silencing of expression of both proteins by RNAi resulted in similar phenotypes, characterized by in...
In this study we sought to identify the cellular and molecular mechanisms underlying presynaptic specificity, with a focus on two unresolved questions: (i) how does a neuron establish functionally distinct presynapses with specific postsynaptic targets, and (ii) how does a presynaptic neuron generate...
Thirty mL of each solution was aseptically transferred to sterile petri dishes (inner diameter 15.6 cm; Sarstedt Ltd., Leicester, UK) and the films were cast (37 °C for 15 h) in a ventilated incubator (Sanyo Ltd., Japan). Dry films were peeled intact and conditioned at room (25 ± ...
Caco-2 and T84 cells were grown in six-well tissue culture dishes. Cells were treated with pathogens with or without probiotic bacteria and lysed with lysis buffer [10 mM HEPES pH 7.6, 150 mM NaCl, 1.5 mM MgCl2, 10 mM KCl, 1 mM EDTA, 1 mM EGTA, 4 mM Na3VO4, 40 mM NaF, ...
In general, the invention relates to screening methods for identifying pathogen virulence factors expressed under conditions of low oxygen and for identifying drugs that inhibit a pathogen. The method includes the steps of: (a) exposing a nematode to a mutagenized pathogen cultured under a low oxyg...
Human NEC (nasal epithelial cells ''RPMI 2650'' from American Type Culture Collection (ATCC), Manassas, VA) were maintained on 100 mm tissue culture dishes, submerged in 10 ml/dish DMEM high glucose supplemented with 10% FBS, 1 Â penicillin–streptomycin solution, and incubated at 371C/...
FINISHED OR IN-PROCESS FOODS SUCH AS CHICKEN, FISH, MEAT IN PLANT OR IN MARKET; FROZEN FOODS DURING PROCESSING, STORAGE, AND SALE; DAIRY UTENSILS, EQUIPMENT, AND CONTAINERS; BULK PASTEURIZING MACHINERY FOR MILK AND BEER; UTENSILS; GLASSWARE, DISHES AND SURFACES IN RESTAURANTS, BARS, HOSPITALS,...