Geobacillus and Parageobacillus are two ecologically diverse thermophilic genera within the phylum Firmicutes. These taxa have long been of biotechnological interest due to their ability to secrete thermostable enzymes and other biomolecules that have di
plates were verified by PCR using the primers trpRtest-F and trpRtest-R, tnaAtest-F and tnaAtest-R, and ptsGtest-F and ptsGtest-R separately. The chlorampenicol or kanamycin cassette was removed with the helper plasmid pCP20. The final strainE. coliK-12 W3110 with three mutations (...
The core secretome was largely dominated by proteins in the COG functional categories of cell wall biogenesis and sporulation, proteins involved in nutrient scavenging and transport across the cell wall/membrane, and proteins of unknown function. Cell wall biogenesis/sporulation proteins include several c...