请问在构建质粒时,质粒上带有factor Xa site,构建时将它切掉了对质粒有影响吗?
Activated Factor V (FVa) functions as a membrane-bound cofactor to the enzyme Factor Xa (FXa) in the conversion of prothrombin to thrombin, increasing the catalytic efficiency of FXa by several orders of magnitude. To map regions on FVa that are important for binding of FXa, site-directed ...
The interaction between factors Va and Xa in solution or on membranes resulted in a small decrease in the overall second-order rate constant (ki/Ki) for the inhibition reaction. The incorporation of DEGRck into the active site of factor Xa results in a large change in the fluorescence ...
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(properly stored) 12 months HCXA-EGR Human Factor Xa, EGRck active site blocked Pricing: Please Inquire SAFETY DATA SHEET Size 100 µg Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4 Storage -80°C Purity >95% by SDS-PAGE Activity Determination Factor X clotting assay or chromogenic assay...
请问在构建质粒时,质粒上带有factor Xa site,构建时将它切掉了对质粒有影响吗?
Substrate Phage Display—To establish the substrate phage display system, we first constructed a positive control recombinant phage pC-Xa (see Fig. 1) with a 7-residue flexible linker between the His6 tag and the fXa cutting site. The flexible linker extended the substrate sequence (IEGR) away...
We present here the crystal structure of the factor Xa·DX-9065a complex. The inhibitor binds in the active site in an extended conformation, which was expected from earlier studies (14, 15). Both hydrophobic and electrostatic interactions characterize the complex formation, which is also accompani...
HCXA-BEGR Human Factor Xa, BEGRck active site blocked HCXA-BEGR由上海拜力生物科技有限公司供应,该产品简介:Coagulation Factor Xa PRODUCT INQUIRY Participation of Factor Xa in Prothrombinase The participati...
2) factor Xa 凝血因子FXa 1. Method The 5′ end of antibacterial peptides gene X was modified by PCR and the cleavage site of factor Xa was used. 目的:研究含凝血因子FXa识别位点的基因与表达载体pGEX KG连接后在大肠杆菌中的表达。3) FX:C 血浆凝血因子X活性 1. Plasma FX:C was tested ...