The power factor of the plant is 70%. The true power being used by the plant is Sign in to download full-size image FIGURE 9-18. Determining the kVAR. kW=kVA×0.70=960kVA×0.70=672kW The plant wishes to increase production by 30%. This would put an additional 201.6 kW load on the...
After four rounds of panning against IsdB NEAT1 domains, the binding of 960 individual phage clones against IsdB and its variants was evaluated by ELISA. About 90% of the clones showed specific binding to full-length IsdB and IsdB NEAT1 domain. Sequence analysis determined that three of the ...
aWe devised a pattern-learning approach for AD classification (partial least squares discriminant analysis, PLS-DA), and trained a dedicated model for each cell-type to distinguish between cells originating from brain tissue of persons with and without AD. Each cell-type-specific model was estimated...
aWe devised a pattern-learning approach for AD classification (partial least squares discriminant analysis, PLS-DA), and trained a dedicated model for each cell-type to distinguish between cells originating from brain tissue of persons with and without AD. Each cell-type-specific model was estimated...
For each such value of R*BP compute ξ0 from Eq. (149) and ReBP from Eqs. (147) and (148). 6. From the computed value of ReBP and the assumed value of R*BP compute f′ from Eq. (138). 7. Repeat steps 4–6 as many times as desired and plot the pairs of points f′, ...
Transcription factors (TFs) play key roles in the regulation of gene expression by binding in a sequence-specific manner to genomic DNA. In eukaryotes, DNA binding is achieved by a wide range of structural forms and motifs. TFs are typically classified b
Gel mobility shift assays The probes used in gel shift assays were obtained by PCR amplification using the oligonucleotide pairs shown in Additional file 2. The double-stranded probes were end-labeled with ( 32P)-ATP using T4 polynucleotide kinase enzyme (Invitrogen, California USA). Gel shift ...
Tissue volume was determined using intrinsic fluorescence with 960 nm excitation and 600–50 nm band pass filter detection. The SHG signal was collected in reflection: the specimen was placed on a deep-well slide, and a mirror was placed underneath to improve collection efficiency. The imaging ...
between two species, we have calculated the ChIP-seq read counts in the promoter of their homologous gene pairs. The conserved GLK target gene pairs have high ChIP-seq signal in both species, while the species-specific ones only have a high ChIP-seq signal in one species (Supplementary Fig....
Additionally, pairs of oligonucleotide primers can be prepared for use in a polymerase chain reaction (PCR) to selectively clone an encoding nucleic acid molecule. A PCR denature/anneal/extend cycle for using such PCR primers is well known in the art and can readily be adapted for use in is...