Transcription factors (TFs) play key roles in the regulation of gene expression by binding in a sequence-specific manner to genomic DNA. In eukaryotes, DNA binding is achieved by a wide range of structural forms and motifs. TFs are typically classified b
Effect of natural genetic variation on enhancer selection and function. Nature 503, 487–492 (2013). Article ADS CAS PubMed PubMed Central Google Scholar Stone, J. R. & Wray, G. A. Rapid evolution of cis-regulatory sequences via local point mutations. Mol. Biol. Evol. 18, 1764–1770...
aSchematic diagram ofBBX18alleles. There were two nucleotide changes in the coding region between SpBBX18 from wild tomato (S. pennelli) and SlBBX18 from cultivated tomato species (S. lycopersicum). The C328T change would lead to an amino acid residue substitution at position 110 from prolin...
2). In addition, Western blot analysis of 12 pairs of human HCC (T) and corresponding adjacent normal liver tissue (N) revealed that ChREBP protein levels were also increased in most of the tumor samples (Fig. 1f and supplementary Table 3). Finally, the expression of MondoA, a paralog...
(PCR) with the primer pairs 5′-TACACAGCAAATGAATGGCAGAGCTGG-3′ (forward) and 5′-GCGTCTTCCTAGTCACCCTCTGTCTTCTG-3′ (reverse), and cloned into the TA cloning vector yT&A, excised by digestion with SmaI/BglII, and cloned into the SmaI/BglII sites of pFRL2 to generate pFRL2-M-...
To better understand the differential contribution of vTNFRs to specific poxvirus-host interaction pairs and because most vTNFRs to date had been studied individually and under different conditions, we provide a direct comparison of the inhibitory capabilities and binding affinities of all vTNFRs produce...
A total of 25 pairs of eyes were histologically examined, 17/25 mice had AMD-like lesions and 23/25 mice had rd8-asso- ciated lesions (Figure 2d). For AMD-like lesion comparison, 14 of 17 (82.4%) mice showed fewer lesions in the PDGF- CC-treated eyes compared with PBS-injected ...
Barcoded IgG-VH (B640 base pairs (bp)) and IgA-VH (B630 bp) transcript libraries were purified using AMPure XP (Beckman Coulter), quantified using PicoGreen (Thermo Fisher Scientific) and pooled at equimolar amounts. The final multiplexed library pools were subjected to emulsion PCR and ...