Transcriptional regulation exhibits extensive robustness, but human genetics indicates sensitivity to transcription factor (TF) dosage. Reconciling such observations requires quantitative studies of TF dosage effects at trait-relevant ranges, largely lac
Except for those in the negative group, the cells were stained with PE anti-mouse F4/80 and FITC anti-mouse CD206 for 45 min on ice in the dark. Then, the cells were washed with PBS before flow cytometry analysis. For the detection of intracellular proteins, the cells were first fixed...
Cytokine synthesis within individual T cells was determined by co-staining with FITC- and/or PE-conjugated anti-cytokine antibodies. Figure 2(a) shows isotype controls and Figure 2(b) the relative lack of T1 cytokine production in unstimulated T cells treated with Brefeldin A. In fact, few ...
Fig. 2: Zfp90 maintains HSC self-renewal ability and repopulation capacity. a,bAnalysis of apoptotic HSCs (Lin−GFP+c-Kit+Sca-1+CD150+CD48−) inZfp90+/+andZfp90−/−mice via Annexin V/PI (a) and active caspase 3 (b) staining 16 weeks after transplantation.cExamination of HSC ...
Deferoxamine (DFO), a widely utilized HIF-1 inducer, has been investigated in clinical trials involving AD patients and has demonstrated a deceleration of cognitive decline [142,150]. These investigations suggest that augmenting HIF-1 activity holds promise for forestalling neuronal demise and alleviat...
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When multiple displays are a must The ThinkStation P340 SFF is a stylish, compact choice for workstation users in fields like architecture, finance, and health care. With up to three DisplayPort™ ports, this workstation supports up to 11 independent displays. It also boasts ISV certifications...
1.2 PCR产物电泳结果 利用合成的引物对不同群体鸡的基因组DNA进行PCR扩增,得到鸡胰岛素样生 产物在大小上没有差异,如图6所示: 图6IQF—l基因的PCR结果 PCR oflGF·1 Fi96 product gene I DNA M:mX74 江西农业大学硕士论文 1.3 PCR.RFLP-Pst I检测结果 限制性内切酶尸j,I的酶切序列为: 5,一～—c 3’...
150. Kloog I, Stevens RG, Haim A, Portnov BA. Nighttime light level co-distributes with breast cancer incidence worldwide. Cancer Causes Control 2010;21:2059–68.10.1007/s10552-010-9624-4Search in Google Scholar PubMed 151. O’Leary ES, Schoenfeld ER, Stevens RG, Kabat GC, Henderson...
After the last centrifugation, the pellet was resuspended in 85 μl of pellet buffer (10 mM Tris-HCL, ph 8.0, 10 mM EDTA, pH 8.0, 150 mM NaCl) followed by 5 μl of Proteinase K (20 mg/ml) (Invitrogen, Grand Island, NY) and 10 μl 10% SDS (Invitrogen). The samples were ...