Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elementsMacrophage, Distal regulatory elements, Nucleosome positioning, FACT, TRIM33BackgroundCell type-specific use of cis-acting regulatory elements is mediated by the combinatorial activity of ...
subunit binds dna, dislocating the outer wraps from the octamer, destabilizing all dna-histone contacts throughout the nucleosome, and increasing the kinetics of nucleosomal fluctuations and even loss during disruption. the spt16 md domain stabilizes the core even while driving the loss of the ...
elegans. Conserved protein domains according to Pfam and InterPro are indicated: Nlob, N-terminal lobe domain; M24, metallopeptidase family M24; SPT16, FACT complex subunit Spt16p/Cdc68p; Rtt, histone chaperone Rttp106-like; SSRP1,structure-specific recognition protein; HMG, high mobility group...
Requirements for RNA polymerase II preinitiation complex formation in vivo. Elife 8, e43654 (2019). Ferri, F., Petit, V., Barroca, V. & Romeo, P. H. Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements. Epigenetics Chromatin 12, ...
However, how this protein complex is targeted to these two distinct cellular processes remains largely unknown. Here we show that ubiquitylation of the Spt16 subunit of FACT by Rtt101, the cullin subunit of an E3 ubiquitin ligase in Saccharomyces cerevisiae, links FACT to DNA replication. We ...
FACT is a heterodimer of SPT16 and SSRP1, which each contain several conserved regions in the primary structure. The interaction of FACT with nucleosomes induces chromatin remodeling through the combinatorial action of its distinct functional protein regions. However, there is little mechanistic insight...
Importantly, FACT activity requires both of its constituent subunits and is dependent on the highly acidic C terminus of its larger subunit, Spt16. These findings define the mechanism by which Pol II can transcribe through chromatin without disrupting its epigenetic status.Belotserkovskaya...
New mutant versions of yeast FACT subunit Spt16 affect cell integrity. Mol Genet Genomics. 2009; 282 :487–502.O'Donnell AF, Stevens JR, Kepkay R, Barnes CA, Johnston GC, Singer RA . ( 2009 ). New mutant versions of yeast FACT subunit Spt16 affect cell integrity . Mol Genet Genomics...
We find that the FACT subunit, Spt16, is ubiquitylated by San1 (an E3 ubiquitin ligase) and degraded by the 26S proteasome. Enhanced abundance of Spt16 in the absence of San1 impairs transcriptional elongation. Likewise, decreased abundance of Spt16 also reduces transcription. Thus, an ...
Conditional inactivation of the FACT subunit Spt16 results in increased Pol II density, transcription, and TATA-binding protein (TBP) occupancy in the 3' portion of certain coding regions, indicating that FACT suppresses inappropriate initiation from cryptic promoters within coding regions. Conversely, ...