Figure 2.Example FSC Vs. SSC FACS Gating Strategy of Lysed Human Whole Blood Excluding Doublets or Multiplets During data acquisition, multiple cells may stick together, forming doublets or multiplets, and when passing through the laser may be counted as one single event. This will potentially ske...
By gating the population of interest, fluorescence detector settings will account for the autofluorescence and nonspecific binding of these cells. 1 Create a polygon region around the lymphocyte population in the FSC vs SSC plot. • Select the Polygon-Region tool ( ). • Click around the ...
圈门逻辑: A.细胞根据其大小(FSC-A/SSC-A)进行圈门,以排除碎片; B.通过FSC-A/FSC-W和SSC-A/SSC-W排除粘连体,圈出单个细胞; C.圈出CD45+总免疫细胞; D.在CD45+门中,圈出Lin+DRAQ7-细胞群; E.在Lin+DRAQ7-细胞群中,通过Ly6G+圈出中性粒细胞; F.Ly6G-群体中,界定CD64+MerTK+的巨噬细胞,并在...