Originally developed in the late 1960s, flow cytometry is a popular analytical cell-biology technique that utilizes light to count and profile cells in a h...
ofcellviabilityusingDead/Livecellgating*ExpressionofGreenFluorescenceProtein(GFP),ords-Red*DetectionofapoptosiswithTUNELorAnnexin-V*Multicoloridentificationorsortingofbacteria,parasites,neurons,sperm,orRBC’s*Quantitativeanalysisofthenumberofmoleculesofaparticularantigenfoundonthesurfaceofacell(MESF)*Celldivision...
23-13536-00Rev.01MulticolorAnalysisAnalysis23-13536-00Rev.012MulticolorAnalysisStrategies•Plottypes•Hierarchicalgating•Populationhierarchies•BackgatingAnalysis23-13536-00Rev.013UseDifferentPlotTypesHistograms—usinghistogramscanhelpdiscriminatebetweentwopopulations.DotPlot:hardtodiscriminatepopulationsDotPlot:disp...
We pride ourselves on the ability to customize any flow cytometry assays to meet our client’s needs. We have extensive experience in data collection and analysis utilizing both straight-forward and complex gating strategies. Flow Contract Site Laboratory, LLC, as your contract flow lab, can rapid...
muscle contraction, cell signaling and ion-channel gating. Specific flow cytometric probes are available to directly look at ion channel activity, most commonly for calcium, or Ca2+. Intracellular Ca2+ levels regulate numerous cellular processes, including gene expression, cellular reproduction and motili...
Putative OSCs were sorted using a BD Biosciences FACSAria I (Beckton Dickinson, UK) cytometer, after gating the negative controls (Supplementary Fig. S2)58. Data were analysed using the FLOWJO software (FLOWJO LLC, USA). OSC cell culture. FACS-sorted DDX4C25-positive cells were seeded ...
(arising because of apoptosis and/or necrosis - see above for reasons). Unless one is specifically measuring apoptosis, these non-viable cells should be excluded from examination by gating. This is because, for example, they may non-specifically bind a fluorescent secondary antibody, the antigen ...
Plasma cells secreting OVA-specific antibodies (Fig.1eand representative gating strategy in Extended Data Fig.4e) were sorted into single wells of 96-well plates, and antibody variable genes were obtained by RT–PCR followed by Sanger sequencing. We recombinantly expressed two antibodies and found...
(fig. 2b and gating strategy in extended data fig. 6b ). secreted antibodies could be identified by comparing the igg signal of samples with and without vhh addition (extended data fig. 6a ). fig. 2: generation of mouse anti-sars-cov-2 rbd antibodies. a , mouse immunization and ...
Plasma cells secreting OVA-specific antibodies (Fig.1eand representative gating strategy in Extended Data Fig.4e) were sorted into single wells of 96-well plates, and antibody variable genes were obtained by RT–PCR followed by Sanger sequencing. We recombinantly expressed two antibodies and found...